Scalia S
Dipartimento di Scienze Farmaceutiche, Università di Ferrara, Italy.
J Pharm Biomed Anal. 1990;8(3):235-41. doi: 10.1016/0731-7085(90)80032-k.
A rapid and simple procedure for the group separation of the major free and conjugated bile acids of man is described. After initial extraction with Bond Elut C18 cartridges, the analytes are fractionated into the unconjugated, glycine- and taurine-conjugated forms using disposable Bond Elut SAX columns and methanol-acetate buffer eluents. The method is found to be accurate and reproducible and to afford complete resolution between fractions. Free and conjugated bile acids present in human bile and gastric juice are assayed by high-performance liquid chromatography, after extraction and group separation according to the described procedure.
本文描述了一种快速简便的方法，用于对人主要游离和结合胆汁酸进行分组分离。先用Bond Elut C18柱进行初步萃取，然后使用一次性Bond Elut SAX柱和甲醇 - 醋酸缓冲洗脱液，将分析物分离为未结合型、甘氨酸结合型和牛磺酸结合型。该方法准确、可重复，各组分之间能实现完全分离。按照所述方法进行萃取和分组分离后，采用高效液相色谱法对人胆汁和胃液中的游离及结合胆汁酸进行测定。
