采用离子对反相液相色谱-电化学检测法测定24小时尿碘排泄量。

Excretion of iodide in 24-h urine as determined by ion-pair reversed-phase liquid chromatography with electrochemical detection.

作者信息

Odink J, Bogaards J J, Sandman H, Egger R J, Arkesteyn G A, de Jong P

机构信息

Department of Clinical Biochemistry, TNO-CIVO Toxicology and Nutrition Institute, Zeist, The Netherlands.

出版信息

J Chromatogr. 1988 Oct 14;431(2):309-16. doi: 10.1016/s0378-4347(00)83099-6.

DOI:10.1016/s0378-4347(00)83099-6

PMID:3243786

Abstract

A simple method is presented for the routine analysis of iodide in urine. After a one-step sample clean-up, iodide was separated by ion-pair reversed-phase liquid chromatography and detected electrochemically with a silver electrode. The coefficient of variation of a single analysis of iodide in a pooled urine sample (530 nmol/l) was 7.6%. The detection limit, derived from a signal-to-noise ratio of 3, was 3 pmol, corresponding to 0.06 mumol/l. The recovery of iodide added to urine was 96 +/- 7%. The accuracy of the method was assessed by analysing ten different samples with neutron activation analysis. The data obtained with the two methods showed a high correlation (r = 0.991) and did not differ significantly. Excretion of iodide in samples of 24-h urine from a free-living population was shown to have a log-normal distribution and to be higher in men than in women. The iodide/creatinine ratio was independent of sex and increased with age.

摘要

本文介绍了一种用于尿液中碘化物常规分析的简单方法。经过一步样品净化后，通过离子对反相液相色谱法分离碘化物，并用银电极进行电化学检测。混合尿液样品（530 nmol/l）中碘化物单次分析的变异系数为7.6%。由信噪比为3得出的检测限为3 pmol，相当于0.06 μmol/l。添加到尿液中的碘化物回收率为96±7%。通过中子活化分析对十个不同样品进行分析来评估该方法的准确性。两种方法获得的数据显示出高度相关性（r = 0.991），且无显著差异。来自自由生活人群的24小时尿液样本中碘化物的排泄呈对数正态分布，男性高于女性。碘化物/肌酐比值与性别无关，且随年龄增加而升高。