快速、特异的高效液相色谱法测定尿中碘化物

Rapid and specific high-performance liquid chromatographic method for the determination of iodide in urine.

作者信息

Mura P, Papet Y, Sanchez A, Piriou A

机构信息

Laboratoire de Biochimie, Hôpital Jean Bernard, Poitiers, France.

出版信息

J Chromatogr B Biomed Appl. 1995 Feb 17;664(2):440-3. doi: 10.1016/0378-4347(94)00486-o.

DOI:10.1016/0378-4347(94)00486-o

PMID:7780600

Abstract

A rapid and specific method for the determination of iodide in urine by high-performance liquid chromatography on an anion-exchange column with electrochemical detection is described. The assay is reproducible as judged by the coefficient of variation of less than 4% at all concentrations used. The limit of detection was 0.1 mumol, and the calibration graph was linear for concentrations between 0.1 and 200 mumol. Using this method, healthy volunteers were found to excrete 69 +/- 39 mumol of iodide per mole of creatinine.

摘要

本文描述了一种采用阴离子交换柱高效液相色谱法结合电化学检测快速、特异性测定尿液中碘化物的方法。在所使用的所有浓度下，变异系数均小于4%，表明该检测方法具有可重复性。检测限为0.1 μmol，在0.1至200 μmol浓度范围内校准曲线呈线性。使用该方法发现，健康志愿者每摩尔肌酐排泄69±39 μmol碘化物。