Kaboosaya Boosana, Wulansari Lia Kartika, Trang Nguyen V N, Kasugai Shohei
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Chulalongkorn University, 254 Phayathai Road, Bangkok, 10330, Thailand.
Department of Prosthodontic, Faculty of Dentistry, University of Indonesia, JI. Salembaraya No. 4, Jakarta Pusat, 10430, Indonesia.
J Oral Biosci. 2020 Jun;62(2):162-168. doi: 10.1016/j.job.2020.04.002. Epub 2020 May 8.
It has been reported that green tea exerts antibacterial, anti-inflammatory, and antioxidant effects. The purpose of the present study was to evaluate the effects of drinking green tea on bone resorption in ligature-induced periodontitis in mice.
Sixty C57BL/6 eight-week-old male mice were used. To induce periodontitis, a ligature was placed for 7 days around the upper left second maxillary molar. After ligature removal, the animals were administered different concentrations of green tea (1.5 g/60 mL, 3 g/60 mL, or 6 g/60 mL) or distilled water. At 1 and 2 weeks of administration, the animals were sacrificed and micro-CT images of the maxillae were taken. Next, the depth and area of alveolar bone loss in the buccal and palatal sides were measured. The number of inflammatory cells and osteoclasts in histological sections were counted.
The result showed ligature-induced alveolar bone loss. Green tea inhibited ligature-induced bone loss in the buccal side in a dose-dependent manner. Histologically, ligature increased the number of inflammatory cells and osteoclasts, but this effect was alleviated by green tea.
Evidence from this animal experiment suggested that drinking green tea would be potentially beneficial to reduce alveolar bone loss in ligature-induced periodontitis.
据报道，绿茶具有抗菌、抗炎和抗氧化作用。本研究的目的是评估饮用绿茶对结扎诱导的小鼠牙周炎骨吸收的影响。
使用60只8周龄的C57BL/6雄性小鼠。为诱导牙周炎，在上颌左第二磨牙周围放置结扎线7天。结扎线去除后，给动物施用不同浓度的绿茶（1.5 g/60 mL、3 g/60 mL或6 g/60 mL）或蒸馏水。给药1周和2周后，处死动物并拍摄上颌骨的微型计算机断层扫描（micro-CT）图像。接下来，测量颊侧和腭侧牙槽骨吸收的深度和面积。对组织学切片中的炎性细胞和破骨细胞数量进行计数。
结果显示结扎诱导牙槽骨吸收。绿茶以剂量依赖性方式抑制结扎诱导的颊侧骨吸收。组织学上，结扎增加了炎性细胞和破骨细胞的数量，但绿茶减轻了这种作用。
该动物实验的证据表明，饮用绿茶可能有利于减少结扎诱导的牙周炎中的牙槽骨吸收。
