Kaczmarska Magdalena, Grosicki Marek, Bulat Katarzyna, Mardyla Mateusz, Szczesny-Malysiak Ewa, Blat Aneta, Dybas Jakub, Sacha Tomasz, Marzec Katarzyna M
Jagiellonian Center for Experimental Therapeutics, Jagiellonian University, Krakow, Poland.
Jagiellonian Center for Experimental Therapeutics, Jagiellonian University, Krakow, Poland; Faculty of Motor Rehabilitation, University of Physical Education, Krakow, Poland.
Nanomedicine. 2020 Aug;28:102221. doi: 10.1016/j.nano.2020.102221. Epub 2020 May 8.
Based on the multimodal characterization of human red blood cells (RBCs), the link between the storage-related sequence of the nanoscale changes in RBC membranes in the relation to their biochemical profile as well as mechanical and functional properties was presented. On the background of the accumulation of RBCs waste products, programmed cell death and impaired rheological properties, progressive alterations in the RBC membranes including changes in their height and diameter as well as the in situ characterization of RBC-derived microparticles (RMPs) on the RBCs surface were presented. The advantage of atomic force microscopy (AFM) in RMPs visualization, even at the very early stage of vesiculation, was shown based on the results revealed by other reference techniques. The nanoscale characterization of RMPs was correlated with a decrease in cholesterol and triglycerides levels in the RBC membranes, proving the link between the lipids leakage from RBCs and the process of vesiculation.
基于对人类红细胞（RBCs）的多模态表征，阐述了红细胞膜纳米级变化的储存相关序列与其生化特征以及机械和功能特性之间的联系。在红细胞废物积累、程序性细胞死亡和流变学特性受损的背景下，介绍了红细胞膜的渐进性改变，包括其高度和直径的变化以及红细胞表面红细胞衍生微粒（RMPs）的原位表征。基于其他参考技术揭示的结果，展示了原子力显微镜（AFM）在RMPs可视化方面的优势，即使在囊泡形成的非常早期阶段。RMPs的纳米级表征与红细胞膜中胆固醇和甘油三酯水平的降低相关，证明了红细胞脂质泄漏与囊泡形成过程之间的联系。
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