Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin, 150500, China; Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region, School of Life Sciences, Heilongjiang University, Harbin, 150080, China; Northeast Forestry University, Harbin, 150040, China.
Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin, 150500, China; Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region, School of Life Sciences, Heilongjiang University, Harbin, 150080, China.
Chemosphere. 2020 Oct;256:127046. doi: 10.1016/j.chemosphere.2020.127046. Epub 2020 May 12.
Although it was well known that arbuscular mycorrhizal fungus (AMF) inoculation significantly increased atrazine dissipation in the soil, the effect of AMF on bacterial community, especially potential atrazine-degrading bacteria mediating atrazine dissipation has been overlooked. In the present study, there were four different treatments: Funnelliformis mosseae inoculation with or without atrazine; and non-AMF inoculation with or without atrazine. F. mosseae significantly increased atrazine dissipation rate from 28.7% to 53.3%. Then 16S rRNA gene sequencing results indicated that bacteria community differed significantly by F. mosseae inoculation and atrazine addition. The Shannon index decreased significantly with AMF and atrazine at phylum and family level, and significant inhibition of atrazine on evenness was also observed. LEFSe analysis revealed that Terrimonas and Arthrobacter were significantly associated with F. mosseae, as well as unidentified_Nitrospiraceae associated with atrazine addition. There are several bacterial taxa associated with both F. mosseae inoculation and atrazine addition. Totally, twelve atrazine-degrading bacterial genera (>0.10%) were identified. When atrazine was added, the abundance of Arthrobacter, Burkholderia, Mycobacterium and Streptomyces increased in F. mosseae inoculation treatment, but Nocardioides, Pseudomonas, Bradyrhizobium, Rhizobium, Rhodobacter, Methylobacterium, Bosea and Shinella decreased. In the presence of atrazine, activities of dehydrogenase, urease, acid and alkaline phosphatase in F. mosseae inoculation treatment were significantly higher than those in non-inoculation. However, there was no significant relationship between bacterial community and any soil enzyme activity in four treatments. Our findings reveal the potential relationship between soil bacterial community and AMF inoculation during atrazine dissipation.
虽然众所周知，丛枝菌根真菌（AMF）接种显著增加了土壤中莠去津的消解，但 AMF 对细菌群落的影响，特别是介导莠去津消解的潜在莠去津降解细菌，一直被忽视。在本研究中，有四种不同的处理：接种摩西管柄囊霉（Funnelliformis mosseae）并添加莠去津或不添加；不接种 AMF 并添加莠去津或不添加。摩西管柄囊霉显著提高了莠去津的消解速率，从 28.7%增加到 53.3%。然后 16S rRNA 基因测序结果表明，细菌群落因摩西管柄囊霉接种和莠去津添加而显著不同。在门和科水平上，摩西管柄囊霉和莠去津的添加显著降低了细菌群落的 Shannon 指数，并且均匀度也受到莠去津的显著抑制。LEFSe 分析表明，Terrimonas 和 Arthrobacter 与摩西管柄囊霉显著相关，而与添加莠去津相关的未鉴定_Nitrospiraceae。有几个与摩西管柄囊霉接种和莠去津添加都相关的细菌类群。总共鉴定出 12 个（>0.10%）莠去津降解细菌属。当添加莠去津时，在摩西管柄囊霉接种处理中，Arthrobacter、Burkholderia、Mycobacterium 和 Streptomyces 的丰度增加，但 Nocardioides、Pseudomonas、Bradyrhizobium、Rhizobium、Rhodobacter、Methylobacterium、Bosea 和 Shinella 的丰度降低。在添加莠去津的情况下，摩西管柄囊霉接种处理中脱氢酶、脲酶、酸和碱性磷酸酶的活性显著高于非接种处理。然而，在四种处理中，细菌群落与任何土壤酶活性之间都没有显著关系。我们的研究结果揭示了在莠去津消解过程中土壤细菌群落与 AMF 接种之间的潜在关系。
