Department of Biological Engineering, College of Life Science, Dalian Minzu University, Dalian 116600, China.
School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230031, China.
Food Chem. 2020 Oct 15;327:127002. doi: 10.1016/j.foodchem.2020.127002. Epub 2020 May 7.
This study was designed to investigate proteome changes in Japanese puffer fish (Takifugu rubripes) during short- and long-term frozen storage. In total, 1484 proteins were quantified, and 164 proteins were identified as differential abundance proteins (DAPs) in Japanese puffer fish from two frozen storage treatment groups (14 days and 60 days) compared with the fresh control group. Correlation analysis between the DAPs and quality traits of the puffer fish muscle showed that 106 proteins were correlated closely with colour and texture (hardness, elasticity, and chewiness). Bioinformatics analysis revealed and Western blot analysis verified that Putative prothymosin alpha species, Bridging integrator 3, NADH: the ubiquinone oxidoreductase subunit and Mx species are candidate biomarkers for puffer fish properties. This study offers valuable evidence to improve the quality control and monitoring of Japanese puffer fish during transportation and storage.
本研究旨在探究日本河豚（Takifugu rubripes）在短期和长期冷冻储存过程中的蛋白质组变化。共定量了 1484 种蛋白质，与新鲜对照组相比，来自两个冷冻储存处理组（14 天和 60 天）的日本河豚中有 164 种蛋白质被鉴定为差异丰度蛋白（DAP）。DAP 与河豚肌肉品质特性的相关性分析表明，106 种蛋白质与颜色和质地（硬度、弹性和咀嚼性）密切相关。生物信息学分析表明，推测的前胸腺素α种、桥连整合因子 3、NADH：泛醌氧化还原酶亚基和 Mx 种是河豚特性的候选生物标志物。本研究为改善日本河豚在运输和储存过程中的质量控制和监测提供了有价值的证据。
