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基于TMT的定量蛋白质组学揭示了不同区域养殖的太平洋鲍鱼中的蛋白质生物标志物。

TMT-based quantitative proteomics reveals protein biomarkers from cultured Pacific abalone () in different regions.

作者信息

Luan Yimu, Dong Yonghui, Duan Xuyuan, Wang Xiuli, Pang Yue, Li Qingwei, Gou Meng

机构信息

College of Life Science, Liaoning Normal University, Dalian 116081, China.

Lamprey Research Center, Liaoning Normal University, Dalian 116081, China.

出版信息

Food Chem X. 2022 Jun 3;14:100355. doi: 10.1016/j.fochx.2022.100355. eCollection 2022 Jun 30.

DOI:10.1016/j.fochx.2022.100355
PMID:35693453
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9184861/
Abstract

Due to latitude, the growth cycle of abalone in southern China is significantly lower than that in the northern regions. Therefore, it often occurs merchants use southern abalone to disguise as northern abalone. This study aims to explore the differences in the muscle proteome of Pacific abalone () in different regions. A total of 1,569 proteins were detected and 729 proteins were identified as differential abundance proteins (DAPs) in cultured in Northern (Liaoning Province) and Southern (Fujian Province) China. Bioinformatics analysis revealed and Western blot verified that fatty acid synthase, troponin I, calpain small subunit 1, and myosin light chain 6 are candidate biomarkers for abalone cultured in different regions. This study provides a deeper understanding of how to distinguish which region abalone is harvested from to improve abalone quality controls, and prevent food fraud.

摘要

由于纬度原因,中国南方鲍鱼的生长周期明显低于北方地区。因此,经常出现商家用南方鲍鱼伪装成北方鲍鱼的情况。本研究旨在探讨不同地区太平洋鲍鱼( )肌肉蛋白质组的差异。在北方(辽宁省)和南方(福建省)养殖的 中,共检测到1569种蛋白质,其中729种蛋白质被鉴定为差异丰度蛋白质(DAPs)。生物信息学分析表明,脂肪酸合酶、肌钙蛋白I、钙蛋白酶小亚基1和肌球蛋白轻链6是不同地区养殖鲍鱼的候选生物标志物,蛋白质印迹法验证了这一点。本研究有助于更深入地了解如何区分鲍鱼的产地,以改善鲍鱼质量控制,防止食品欺诈。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4408/9184861/69fc821c126f/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4408/9184861/4cc5f857b0e0/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4408/9184861/7b7d42b6e3ef/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4408/9184861/089b4f439a2f/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4408/9184861/407a33a55a6a/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4408/9184861/69fc821c126f/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4408/9184861/4cc5f857b0e0/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4408/9184861/7b7d42b6e3ef/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4408/9184861/089b4f439a2f/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4408/9184861/407a33a55a6a/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4408/9184861/69fc821c126f/gr5.jpg

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