Bargiela Rafael, Lanthaler Karin, Potter Colin M, Ferrer Manuel, Yakunin Alexander F, Paizs Bela, Golyshin Peter N, Golyshina Olga V
School of Natural Sciences, Bangor University, Deiniol Rd, Bangor LL57 2UW, UK.
Centre for Environmental Biotechnology, Bangor University, Deiniol Rd, Bangor LL57 2UW, UK.
Microorganisms. 2020 May 19;8(5):759. doi: 10.3390/microorganisms8050759.
The archaeon is ubiquitous in acidic environments with low-to-moderate temperatures. However, molecular mechanisms underlying its ability to thrive at lower temperatures remain unexplored. Using mass spectrometry (MS)-based proteomics, we analysed the effect of short-term (3 h) exposure to cold. The genome encodes 2016 protein-coding genes, from which 819 proteins were identified in the cells grown under optimal conditions. In line with the peptidolytic lifestyle of , its intracellular proteome revealed the abundance of proteases, ABC transporters and cytochrome C oxidase. From 747 quantifiable polypeptides, the levels of 582 proteins showed no change after the cold shock, whereas 104 proteins were upregulated suggesting that they might be contributing to cold adaptation. The highest increase in expression appeared in low-abundance (0.001-0.005 fmol%) proteins for polypeptides' hydrolysis (metal-dependent hydrolase), oxidation of amino acids (FAD-dependent oxidoreductase), pyrimidine biosynthesis (aspartate carbamoyltransferase regulatory chain proteins), citrate cycle (2-oxoacid ferredoxin oxidoreductase) and ATP production (V type ATP synthase). Importantly, the cold shock induced a substantial increase (6% and 9%) in expression of the most-abundant proteins, thermosome beta subunit and glutamate dehydrogenase. This study has outlined potential mechanisms of environmental fitness of spp. allowing them to colonise acidic settings at low/moderate temperatures.
古菌在低温至中度温度的酸性环境中普遍存在。然而，其在较低温度下茁壮成长的分子机制仍未得到探索。我们使用基于质谱（MS）的蛋白质组学分析了短期（3小时）暴露于寒冷的影响。该基因组编码2016个蛋白质编码基因，在最佳条件下生长的细胞中鉴定出其中819种蛋白质。与其肽解生活方式一致，其细胞内蛋白质组显示出丰富的蛋白酶、ABC转运蛋白和细胞色素C氧化酶。在747种可定量的多肽中，582种蛋白质的水平在冷休克后没有变化，而104种蛋白质上调，表明它们可能有助于冷适应。表达增加最高的是低丰度（0.001 - 0.005 fmol%）的蛋白质，涉及多肽水解（金属依赖性水解酶）、氨基酸氧化（FAD依赖性氧化还原酶）、嘧啶生物合成（天冬氨酸氨甲酰转移酶调节链蛋白）、柠檬酸循环（2-氧代酸铁氧还蛋白氧化还原酶）和ATP产生（V型ATP合酶）。重要的是，冷休克导致最丰富的蛋白质——热体β亚基和谷氨酸脱氢酶的表达大幅增加（分别为6%和9%）。这项研究概述了该古菌种环境适应性的潜在机制，使其能够在低温/中度温度下在酸性环境中定殖。
