Department of Herbal Pharmacology, College of Korean Medicine, Gachon University, 1342 Seongnamdae-ro, Sujeong-gu, Seongnam 13120, Korea.
Korea Institute of Science and Technology for Eastern Medicine (KISTEM) NeuMed Inc., 88 Imun-ro, Dongdaemun-gu, Seoul 02440, Korea.
Nutrients. 2020 May 19;12(5):1477. doi: 10.3390/nu12051477.
Osteoarthritis (OA) is a chronic inflammatory joint disease that affects millions of elderly people around the world. The conventional treatments for OA consisting of nonsteroidal anti-inflammatory drugs and steroid have negative health consequences, such as gastrointestinal, renal, and cardiac diseases. This study has evaluated the Commiphora extract mixture (HT083) on OA progression as an alternative treatment in animal models. The root of and the gum resin of have been in use as traditional medicines against many health problems including bone disorders since ancient time. The extracts of root and gum resin were mixed as 3:1 for their optimal effects. Male Sprague-Dawley rats were injected with monosodium iodoacetate (MIA) into the knee joints to induce the symptoms identical to human OA. HT083 substantially prevented the loss of weight-bearing inflicted with MIA in rats. The MIA-induced cartilage erosion as well as the subchondral bone damage in the rats was also reversed. In addition, the increase of serum IL-1β concentration, a crucial pro-inflammatory cytokine involved in OA progression was countered by HT083. Furthermore, HT083 significantly reduced the acetic acid-induced writhing response in mice. In vitro, HT083 has shown potent anti-inflammatory activities by inhibiting the production of NO and suppressing the interleukin -1β, interleukin -6, cyclooxygenase-2, and inducible nitric oxide synthase expression in lipopolysaccharide -stimulated RAW 264.7 cells. Given its potent analgesic and anti-inflammatory activities in MIA rats and acetic acid-induced writhing in mice, HT083 should be further studied in order to explain its mechanism of actions in alleviating OA pain and inflammation.
骨关节炎（OA）是一种慢性炎症性关节疾病，影响着全世界数以百万计的老年人。OA 的传统治疗方法包括非甾体抗炎药和类固醇，这些方法会带来负面的健康后果，如胃肠道、肾脏和心脏疾病。本研究评估了 Commiphora 提取物混合物（HT083）作为替代治疗方法在动物模型中对 OA 进展的影响。自古以来， 和 的根以及 的胶脂一直被用作治疗许多健康问题的传统药物，包括骨骼疾病。为了达到最佳效果， 将 根和 胶脂的提取物按 3:1 混合。雄性 Sprague-Dawley 大鼠膝关节内注射单碘乙酸盐（MIA），以诱导与人类 OA 相同的症状。HT083 显著防止了 MIA 引起的大鼠体重减轻。MIA 诱导的软骨侵蚀以及软骨下骨损伤也得到了逆转。此外，HT083 还对抗了 MIA 诱导的血清 IL-1β浓度升高，IL-1β 是一种参与 OA 进展的关键促炎细胞因子。此外，HT083 还显著降低了醋酸诱导的小鼠扭体反应。体外实验表明，HT083 通过抑制一氧化氮的产生和抑制白细胞介素-1β、白细胞介素-6、环氧化酶-2 和诱导型一氧化氮合酶在脂多糖刺激的 RAW 264.7 细胞中的表达，具有很强的抗炎活性。鉴于 HT083 在 MIA 大鼠和醋酸诱导的小鼠扭体反应中具有很强的镇痛和抗炎活性，应该进一步研究其在缓解 OA 疼痛和炎症中的作用机制。
