KM Science Research Division, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Daejeon 34054, Korea.
Institute of Traditional Medicine and Bioscience, Daejeon University, Daejeon 34520, Korea.
Nutrients. 2022 Mar 24;14(7):1356. doi: 10.3390/nu14071356.
In this study, we aimed to determine the anti-inflammatory and antinociceptive activities of leaf extracts (SCLE) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages, an acetic acid-induced mouse model of writhing, and a monosodium iodoacetate (MIA)-induced rat model of osteoarthritis (OA). In LPS-stimulated RAW264.7 cells, a 100 µg/mL dose of SCLE significantly reduced the production of nitric oxide (NO), interleukin-1β (IL-1β), tumour necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and prostaglandin E2 (PGE2). Acetic acid-induced writhing responses in mice that quantitatively determine pain were significantly inhibited by SCLE treatment. In addition, SCLE significantly decreased the MIA-induced elevation in OA symptoms, the expression levels of pro-inflammatory mediators/cytokines and matrix metalloproteinases, and cartilage damage in the serum and joint tissues. Our data demonstrated that SCLE exerts anti-osteoarthritic effects by regulating inflammation and pain and can be a useful therapeutic candidate against OA.
在这项研究中，我们旨在确定叶提取物（SCLE）在脂多糖（LPS）刺激的 RAW 264.7 巨噬细胞、乙酸诱导的扭体小鼠模型和碘乙酸单钠（MIA）诱导的骨关节炎（OA）大鼠模型中的抗炎和镇痛活性。在 LPS 刺激的 RAW264.7 细胞中，100 µg/mL 剂量的 SCLE 可显著降低一氧化氮（NO）、白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和前列腺素 E2（PGE2）的产生。SCLE 处理可显著抑制乙酸诱导的扭体反应，定量测定疼痛。此外，SCLE 还可显著降低 MIA 诱导的 OA 症状、促炎介质/细胞因子和基质金属蛋白酶的表达水平以及血清和关节组织中的软骨损伤。我们的数据表明，SCLE 通过调节炎症和疼痛发挥抗骨关节炎作用，可作为治疗 OA 的有效候选药物。
