Liao J L, Hjertén S
Institute of Biochemistry, University of Uppsala, Sweden.
J Chromatogr. 1988 Dec 21;457:175-82. doi: 10.1016/s0021-9673(01)82065-3.
Macroporous agarose beads were rendered impermeable to proteins by shrinkage and cross-linking in organic solvents. The chromatographic properties of compressed beds of these non-porous beads derivatized for high-performance ion-exchange chromatography were studied, e.g., the resolution as a function of gradient time, flow-rate (at constant gradient volume) and loading capacity. The columns permit high flow-rates and the resolution is about the same at low and high flow-rates. The beads are stable up to pH 14.
