High-performance liquid chromatography of proteins on compressed, non-porous agarose beads. II. Anion-exchange chromatography.

Macroporous agarose beads were rendered impermeable to proteins by shrinkage and cross-linking in organic solvents. The chromatographic properties of compressed beds of these non-porous beads derivatized for high-performance ion-exchange chromatography were studied, e.g., the resolution as a function of gradient time, flow-rate (at constant gradient volume) and loading capacity. The columns permit high flow-rates and the resolution is about the same at low and high flow-rates. The beads are stable up to pH 14.