Hjertén S, Liao J L
Institute of Biochemistry, University of Uppsala, Sweden.
J Chromatogr. 1988 Dec 21;457:165-74. doi: 10.1016/s0021-9673(01)82064-1.
Macroporous agarose beads were converted into non-porous beads by shrinkage and cross-linking in organic solvents. These beads could be used for high-performance hydrophobic-interaction chromatography without derivatization with non-polar ligands, because the 1,4-butanediol diglycidyl ether, used as cross-linker, gives relatively hydrophobic bridges. The resolution for compressed columns packed with these beads was determined as a function of gradient time at constant flow-rate, flow-rate at constant gradient volume and flow-rate at constant gradient time and as a function of loading capacity. Interestingly, the resolution is virtually independent of flow-rate at constant gradient volume even when the column is packed with relatively large beads (diameter 30 microns). The beads have the advantage of being stable up to pH 14.
大孔琼脂糖珠通过在有机溶剂中收缩和交联转化为无孔珠。这些珠子可用于高效疏水相互作用色谱，无需用非极性配体进行衍生化，因为用作交联剂的1,4-丁二醇二缩水甘油醚会形成相对疏水的桥。测定了填充这些珠子的压缩柱的分辨率与恒定流速下梯度时间、恒定梯度体积下流速、恒定梯度时间下流速的函数关系以及与负载量的函数关系。有趣的是，即使柱中填充的是相对较大的珠子（直径30微米），在恒定梯度体积下分辨率实际上也与流速无关。这些珠子的优点是在pH值高达14时仍很稳定。
