Michels J J, Dorsey J G
Department of Chemistry, University of Florida, Gainesville 32611.
J Chromatogr. 1988 Dec 21;457:85-98. doi: 10.1016/s0021-9673(01)82057-4.
The mechanism of retention in reversed-phase liquid chromatography (RPLC) has been further investigated using the ET(30) solvatochromic solvent polarity scale. The retention behavior of a variety of solutes was measured using a homologous series of normal alcohols as the organic modifiers in hydroorganic mobile phases. The results imply that a systematic change in the extent of solvation of the stationary phase occurs with respect to the size of the organic modifier. It was also found that a linear extrapolation of the log k' versus ET(30) plots for different mobile phases using methanol, ethanol and acetonitrile (but not n-propanol) as modifiers, intersected at approximately the ET(30) value of pure water. This intersection is further evidence that the ET(30) model of solute retention is a useful tool with which to study the mechanism of retention in RPLC. The extrapolated retention value in water, log k'w, from the ET(30) plots should then prove to be a more reliable means of estimating solute lipophilicity using RPLC than the percent organic modifier model.
采用ET(30)溶剂化显色溶剂极性标度，对反相液相色谱(RPLC)中的保留机理进行了进一步研究。以一系列正构醇作为水-有机流动相中的有机改性剂，测定了多种溶质的保留行为。结果表明，固定相的溶剂化程度随有机改性剂的大小而发生系统性变化。还发现，以甲醇、乙醇和乙腈（而非正丙醇）作为改性剂，不同流动相的log k'与ET(30)曲线的线性外推在纯水的ET(30)值处相交。这一交点进一步证明，溶质保留的ET(30)模型是研究RPLC保留机理的有用工具。那么，从ET(30)曲线得出的水中外推保留值log k'w，应比有机改性剂百分比模型更可靠地用于通过RPLC估算溶质亲脂性。
