State Key Laboratory for Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
Department of Infection Control, Shanghai Pulmonary Hospital, Tongji University, Shanghai 200433, China.
Biomed Environ Sci. 2020 Apr 20;33(4):248-259. doi: 10.3967/bes2020.034.
Our objective was to investigate the occurrence of opportunistic pathogens and characterize the bacterial community structures in the water system of a pulmonary hospital.
The water samples were collected from automatic and manual faucets in the consulting room, treatment room, dressing room, respiratory ward, and other non-medical rooms in three buildings of the hospital. Quantitative polymerase chain reaction was used to quantify the load of several waterborne opportunistic pathogens and related microorganisms, including spp., spp., and . Illumina sequencing targeting 16S rRNA genes was performed to profile bacterial communities.
The occurrence rates of spp., spp., and were 100%, 100%, and 76%, respectively in all samples. Higher occurrence rates of were observed in the outpatient service building (building 1, 91.7%) and respiration department and wards (building 2, 80%) than in the office building (building 3), where no was found. were more abundant in automatic faucets (average 2.21 × 10 gene copies/L) than in manual faucets (average 1.03 × 10 gene copies/mL) ( < 0.01). , , , , , and were the dominant bacterial phyla. Disinfectant residuals, nitrate, and temperature were found to be the key environmental factors driving microbial community structure shifts in water systems.
This study revealed a high level of colonization of water faucets by opportunistic pathogens and provided insight into the characteristics of microbial communities in a hospital water system and approaches to reduce risks of microbial contamination.
本研究旨在调查某家肺病专科医院水系统中机会性病原体的发生情况，并分析其细菌群落结构特征。
从医院三栋建筑中的诊室、治疗室、更衣室、呼吸科病房和其他非医疗用房的自动和手动水龙头中采集水样。采用定量聚合酶链反应（qPCR）定量检测几种水源性机会性病原体及其相关微生物（包括 spp.、 spp.和 ）的负荷量。采用 Illumina 测序技术靶向 16S rRNA 基因对细菌群落进行分析。
所有水样中 spp.、 spp.和 的检出率均为 100%。门诊楼（1 号楼）和呼吸科及其病房（2 号楼）的 检出率（91.7%和 80%）高于办公楼（3 号楼）（未检出 ）。自动水龙头（平均 2.21×10 基因拷贝/L）中 的丰度高于手动水龙头（平均 1.03×10 基因拷贝/L）（ < 0.01）。细菌门水平上， 、 、 、 、 和 为主要的优势菌门。研究发现，消毒剂残留、硝酸盐和温度是驱动水系统微生物群落结构变化的关键环境因素。
本研究揭示了水龙头被机会性病原体定植的高风险，为了解医院水系统中微生物群落的特征以及减少微生物污染风险的方法提供了新视角。
