• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

1 型或 2 型子宫内膜癌研究中内源性对照基因的选择。

Selection of Endogenous Control Reference Genes for Studies on Type 1 or Type 2 Endometrial Cancer.

机构信息

Faculty of Health and Life Sciences, University of Liverpool, Thompson Yates Brownlow Hill, Liverpool, L69 3GB, UK.

Reproductive Sciences Section, Department of Cancer Studies and Molecular Medicine, University of Leicester, Leicester, UK.

出版信息

Sci Rep. 2020 May 21;10(1):8468. doi: 10.1038/s41598-020-64663-4.

DOI:10.1038/s41598-020-64663-4
PMID:32439920
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7242460/
Abstract

A panel of 32 candidate reference genes was used to identify the most stable genes for gene normalisation in quantitative RT-PCR studies using endometrial biopsies obtained from women with endometrial cancer (type 1 or type 2) and without cancer (controls). RNA from the biopsies was isolated, examined for purity and quality, and then reverse transcribed into cDNA before being subjected to real-time qRT-PCR analysis in triplicate within the TaqMan gene Expression Assay kit. The most 'stable' endogenous control genes were then identified using the geNorm qbase + 2 and NormFinder software packages. PSMC4, PUM1 and IPO8 were identified as the best reference genes combination for type 1 endometrial cancer (grades 1, 2 and 3), whereas for type 2 endometrial cancer (serous and carcinosarcoma), UBC, MRPL19, PGK1 and PPIA were the best reference genes combination. We conclude that the use of these normaliser combinations should provide accurate interpretation of gene expression at the transcript level in endometrial cancer studies especially for types 1 and 2 cancers.

摘要

使用 32 个候选参考基因的小组，以鉴定子宫内膜癌（1 型或 2 型）和无癌症（对照组）女性子宫内膜活检中定量 RT-PCR 研究基因正常化的最稳定基因。从活检中分离 RNA，检查纯度和质量，然后反转录为 cDNA，然后在 TaqMan 基因表达分析试剂盒中进行三重复实时 qRT-PCR 分析。然后使用 geNorm qbase+2 和 NormFinder 软件包确定最“稳定”的内参基因。PSMC4、PUM1 和 IPO8 被确定为 1 型子宫内膜癌（1 级、2 级和 3 级）的最佳参考基因组合，而对于 2 型子宫内膜癌（浆液性和癌肉瘤），UBC、MRPL19、PGK1 和 PPIA 是最佳参考基因组合。我们得出结论，这些归一化组合的使用应在子宫内膜癌研究中特别是在 1 型和 2 型癌症中提供转录水平基因表达的准确解释。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ccb/7242460/c82897301da4/41598_2020_64663_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ccb/7242460/476fdf8aa078/41598_2020_64663_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ccb/7242460/521e53c7af01/41598_2020_64663_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ccb/7242460/c82897301da4/41598_2020_64663_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ccb/7242460/476fdf8aa078/41598_2020_64663_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ccb/7242460/521e53c7af01/41598_2020_64663_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ccb/7242460/c82897301da4/41598_2020_64663_Fig3_HTML.jpg

相似文献

1
Selection of Endogenous Control Reference Genes for Studies on Type 1 or Type 2 Endometrial Cancer.1 型或 2 型子宫内膜癌研究中内源性对照基因的选择。
Sci Rep. 2020 May 21;10(1):8468. doi: 10.1038/s41598-020-64663-4.
2
Validation of endogenous control reference genes for normalizing gene expression studies in endometrial carcinoma.用于标准化子宫内膜癌基因表达研究的内参基因的验证
Mol Hum Reprod. 2015 Sep;21(9):723-35. doi: 10.1093/molehr/gav033. Epub 2015 Jun 29.
3
Identification of a novel estrogen-regulated gene, EIG121, induced by hormone replacement therapy and differentially expressed in type I and type II endometrial cancer.一种新型雌激素调节基因EIG121的鉴定，该基因由激素替代疗法诱导，在I型和II型子宫内膜癌中差异表达。
Clin Cancer Res. 2005 Dec 1;11(23):8258-64. doi: 10.1158/1078-0432.CCR-05-1189.
4
Investigating Novel Genes Potentially Involved in Endometrial Adenocarcinoma using Next-Generation Sequencing and Bioinformatic Approaches.利用下一代测序和生物信息学方法研究潜在参与子宫内膜腺癌的新基因。
Int J Med Sci. 2019 Sep 7;16(10):1338-1348. doi: 10.7150/ijms.38219. eCollection 2019.
5
Identification of endogenous reference genes for qRT-PCR analysis in normal matched breast tumor tissues.在正常匹配的乳腺肿瘤组织中用于qRT-PCR分析的内源性参考基因的鉴定。
Oncol Res. 2009;17(8):353-65. doi: 10.3727/096504009788428460.
6
Selection and validation of reliable reference genes for RT-qPCR analysis in a large cohort of pituitary adenomas.垂体腺瘤大样本队列中用于RT-qPCR分析的可靠内参基因的筛选与验证
Mol Cell Endocrinol. 2016 Dec 5;437:183-189. doi: 10.1016/j.mce.2016.08.030. Epub 2016 Aug 22.
7
[Expression profile of genes associated with the histaminergic system estimated by oligonucleotide microarray analysis HG-U133A in women with endometrial adenocarcinoma].[通过寡核苷酸微阵列分析HG-U133A评估子宫内膜腺癌女性中与组胺能系统相关基因的表达谱]
Ginekol Pol. 2014 Mar;85(3):172-9. doi: 10.17772/gp/1709.
8
Identification of optimal reference genes for gene expression normalization in a wide cohort of endometrioid endometrial carcinoma tissues.在大量子宫内膜样子宫内膜癌组织中鉴定用于基因表达标准化的最佳内参基因。
PLoS One. 2014 Dec 4;9(12):e113781. doi: 10.1371/journal.pone.0113781. eCollection 2014.
9
Reference genes for quantitative RT-PCR data in gastric tissues and cell lines.胃组织和细胞系定量 RT-PCR 数据的参考基因。
World J Gastroenterol. 2013 Nov 7;19(41):7121-8. doi: 10.3748/wjg.v19.i41.7121.
10
Expression stability of common housekeeping genes is differently affected by bowel inflammation and cancer: implications for finding suitable normalizers for inflammatory bowel disease studies.常见看家基因的表达稳定性受肠道炎症和癌症的影响不同：对寻找炎症性肠病研究中合适的标准化基因的启示
Inflamm Bowel Dis. 2014 Jul;20(7):1147-56. doi: 10.1097/MIB.0000000000000067.

引用本文的文献

1
Selecting Optimal Housekeeping Genes for RT-qPCR in Endometrial Cancer Studies: A Narrative Review.子宫内膜癌研究中用于逆转录定量聚合酶链反应的最佳管家基因选择：一项叙述性综述
Int J Mol Sci. 2025 Sep 4;26(17):8610. doi: 10.3390/ijms26178610.
2
ciRS-7 expression is epigenetically regulated in cancer cells across human adenocarcinomas.在人类腺癌的癌细胞中，ciRS-7的表达受表观遗传调控。
PLoS Genet. 2025 Jun 2;21(6):e1011726. doi: 10.1371/journal.pgen.1011726. eCollection 2025 Jun.
3
The role of PSMC4 in non-small cell lung cancer: implications for prognosis, diagnosis, and immune microenvironment modulation.

本文引用的文献

1
Reprint of: Dating the Endometrial Biopsy.《子宫内膜活检的时间判定》重印版
Fertil Steril. 2019 Oct;112(4 Suppl1):e93-e115. doi: 10.1016/j.fertnstert.2019.08.079.
2
Pan-Cancer Analysis of TCGA Data Revealed Promising Reference Genes for qPCR Normalization.基于TCGA数据的泛癌分析揭示了用于qPCR标准化的有前景的参考基因。
Front Genet. 2019 Mar 1;10:97. doi: 10.3389/fgene.2019.00097. eCollection 2019.
3
Endometrial Cancer: Obesity, Genetics, and Targeted Agents.子宫内膜癌：肥胖、遗传与靶向药物。
PSMC4在非小细胞肺癌中的作用：对预后、诊断及免疫微环境调节的意义
Front Oncol. 2025 May 2;15:1503466. doi: 10.3389/fonc.2025.1503466. eCollection 2025.
4
KT-253, a Novel MDM2 Degrader and p53 Stabilizer, Has Superior Potency and Efficacy than MDM2 Small-Molecule Inhibitors.新型MDM2降解剂及p53稳定剂KT-253比MDM2小分子抑制剂具有更强的效力和疗效。
Mol Cancer Ther. 2025 Apr 2;24(4):497-510. doi: 10.1158/1535-7163.MCT-24-0306.
5
The PPP2R1A cancer hotspot mutant p.R183W increases clofarabine resistance in uterine serous carcinoma cells by a gain-of-function mechanism.PPP2R1A 癌症热点突变 p.R183W 通过获得性功能机制增加子宫浆液性癌细胞对氯法拉滨的耐药性。
Cell Oncol (Dordr). 2024 Oct;47(5):1811-1829. doi: 10.1007/s13402-024-00963-5. Epub 2024 Jun 18.
6
EndoGeneAnalyzer: A tool for selection and validation of reference genes.EndoGeneAnalyzer：一个用于选择和验证参考基因的工具。
PLoS One. 2024 Apr 3;19(4):e0299993. doi: 10.1371/journal.pone.0299993. eCollection 2024.
7
Validation of Endogenous Control Genes by Real-Time Quantitative Reverse Transcriptase Polymerase Chain Reaction for Acute Leukemia Gene Expression Studies.实时定量逆转录聚合酶链反应验证内参基因在急性白血病基因表达研究中的应用。
Genes (Basel). 2024 Jan 24;15(2):151. doi: 10.3390/genes15020151.
8
Fer-mediated activation of the Ras-MAPK signaling pathway drives the proliferation, migration, and invasion of endometrial carcinoma cells.铁介导的 Ras-MAPK 信号通路的激活驱动子宫内膜癌细胞的增殖、迁移和侵袭。
Mol Cell Biochem. 2024 Jul;479(7):1787-1799. doi: 10.1007/s11010-023-04890-1. Epub 2023 Nov 28.
9
A novel APA-based prognostic signature may predict the prognosis of lung adenocarcinoma in an East Asian population.一种基于新型APA的预后特征可能预测东亚人群肺腺癌的预后。
iScience. 2023 Sep 28;26(11):108068. doi: 10.1016/j.isci.2023.108068. eCollection 2023 Nov 17.
10
Reducing the Invasiveness of Low- and High-Grade Endometrial Cancers in Both Primary Human Cancer Biopsies and Cell Lines by the Inhibition of Aquaporin-1 Channels.通过抑制水通道蛋白-1通道降低原发性人类癌症活检组织和细胞系中低级别和高级别子宫内膜癌的侵袭性
Cancers (Basel). 2023 Sep 11;15(18):4507. doi: 10.3390/cancers15184507.
Obstet Gynecol Clin North Am. 2019 Mar;46(1):89-105. doi: 10.1016/j.ogc.2018.09.006.
4
Cannabinoid receptor expression in estrogen-dependent and estrogen-independent endometrial cancer.雌激素依赖性和非雌激素依赖性子宫内膜癌中大麻素受体的表达
J Recept Signal Transduct Res. 2018 Oct-Dec;38(5-6):385-392. doi: 10.1080/10799893.2018.1531890. Epub 2018 Dec 20.
5
RNA Biomarkers: Frontier of Precision Medicine for Cancer.RNA生物标志物：癌症精准医学的前沿领域。
Noncoding RNA. 2017 Feb 20;3(1):9. doi: 10.3390/ncrna3010009.
6
Towards precision medicine: discovering novel gynecological cancer biomarkers and pathways using linked data.迈向精准医学：利用关联数据发现新型妇科癌症生物标志物和通路。
J Biomed Semantics. 2017 Sep 19;8(1):40. doi: 10.1186/s13326-017-0146-9.
7
The genetic regulation of transcription in human endometrial tissue.人类子宫内膜组织中转录的遗传调控。
Hum Reprod. 2017 Apr 1;32(4):893-904. doi: 10.1093/humrep/dex006.
8
Validation of endogenous control reference genes for normalizing gene expression studies in endometrial carcinoma.用于标准化子宫内膜癌基因表达研究的内参基因的验证
Mol Hum Reprod. 2015 Sep;21(9):723-35. doi: 10.1093/molehr/gav033. Epub 2015 Jun 29.
9
Identification of optimal reference genes for gene expression normalization in a wide cohort of endometrioid endometrial carcinoma tissues.在大量子宫内膜样子宫内膜癌组织中鉴定用于基因表达标准化的最佳内参基因。
PLoS One. 2014 Dec 4;9(12):e113781. doi: 10.1371/journal.pone.0113781. eCollection 2014.
10
Selection and validation of endogenous controls for microRNA expression studies in endometrioid endometrial cancer tissues.选择和验证内参基因用于子宫内膜样型子宫内膜癌组织中 microRNA 表达研究。
Gynecol Oncol. 2013 Sep;130(3):588-94. doi: 10.1016/j.ygyno.2013.06.026. Epub 2013 Jun 26.