Eadsforth C V, Coveney P C, Sjoe W H
Shell International Petroleum Maatschappij B.V., Health, Safety and Environmental Division, The Hague, The Netherlands.
J Anal Toxicol. 1988 Nov-Dec;12(6):330-3. doi: 10.1093/jat/12.6.330.
An HPLC method for monitoring exposure of workers to 3-chloro-4-fluoroaniline (CFA) is described. It is based on the detection of a major urinary metabolite, 2-amino-4-chloro-5-fluorophenyl sulphate, and is superior to the previously adopted GC method. The limit of detection for the metabolite, 2-amino-4-chloro-5-fluorophenyl sulphate, is less than or equal to 0.01 mg/L (as CFA equivalent), with a coefficient of variation obtained over the range 1-15 mg/L of 4%. The HPLC method avoids the need for derivatization (cf. GC) and is consequently a much quicker and cheaper monitoring method. Urine samples from plant workers may be stored at 4 degrees C for a period of three months without loss of the metabolite.
本文描述了一种用于监测工人接触3-氯-4-氟苯胺（CFA）情况的高效液相色谱法。该方法基于对主要尿代谢物2-氨基-4-氯-5-氟苯基硫酸盐的检测，优于先前采用的气相色谱法。代谢物2-氨基-4-氯-5-氟苯基硫酸盐的检测限小于或等于0.01 mg/L（以CFA当量计），在1 - 15 mg/L范围内获得的变异系数为4%。高效液相色谱法无需衍生化（与气相色谱法相比），因此是一种更快且更便宜的监测方法。工厂工人的尿液样本可在4℃下储存三个月，而不会导致代谢物损失。
