Li Jianwu, Li Maoyin, Yao Shuaibing, Cai Guangqin, Wang Xuemin
Henan Agricultural University, Zhengzhou, Henan 450002, China.
Department of Biology, University of Missouri, St. Louis, MO 63121, USA.
Plants (Basel). 2020 May 20;9(5):650. doi: 10.3390/plants9050650.
Patatinrelated phospholipases (pPLAs) are acylhydrolyzing enzymes implicated in various processes, including lipid metabolism, signal transduction, plant growth and stress responses, but the function for many specific pPLAs in plants remains unknown. Here we determine the effect of patatinrelated phospholipase A pPLAIIIγ on response to abiotic stress. Knockout of rendered plants more sensitive whereas overexpression of enhanced plant tolerance to NaCl and drought in seed germination and seedling growth. The knockout and overexpressing seedlings displayed a lower and higher level of lysolipids and free fatty acids than that of wildtype plants in response to NaCl stress, respectively. These results indicate that pPLAIIIγ acts a positive regulator of salt and osmatic stress tolerance in .
类Patatin磷脂酶(pPLAs)是参与各种过程的酰基水解酶,包括脂质代谢、信号转导、植物生长和胁迫反应,但许多特定pPLAs在植物中的功能仍不清楚。在这里,我们确定了类Patatin磷脂酶A pPLAIIIγ对非生物胁迫响应的影响。pPLAIIIγ基因敲除使植物更敏感,而pPLAIIIγ过表达增强了植物在种子萌发和幼苗生长过程中对NaCl和干旱的耐受性。在NaCl胁迫下,基因敲除和过表达的幼苗分别比野生型植物表现出更低和更高水平的溶血磷脂和游离脂肪酸。这些结果表明,pPLAIIIγ在植物中作为盐和渗透胁迫耐受性的正调控因子发挥作用。