磷脂酸抑制 SCAB1 介导的 F-actin 束形成。
Phosphatidic acid inhibits SCAB1-mediated F-actin bundling in .
机构信息
College of Biological Sciences, China Agricultural University, Beijing, Haidian, China.
出版信息
Plant Signal Behav. 2023 Dec 31;18(1):2092346. doi: 10.1080/15592324.2022.2092346. Epub 2022 Jun 26.
Stomatal closure-associated actin-binding protein 1 (SCAB1) regulates stomatal closure by mediating actin filament reorganization in . Our previous study showed that phosphatidylinositol 3-phosphate (PI3P) binds to SCAB1 and inhibits its oligomerization, thereby inhibiting its activity on F-actin in guard cells during stomatal closure. In this study, we show that another phospholipid, phosphatidic acid (PA), also binds to SCAB1 and inhibits its actin-bundling activity but not its actin-binding activity. F-actin bundling was promoted by treating Col-0 seedlings with -butanol, a suppressor of PA production, but this effect was absent in the mutant. These results indicate that the signaling molecule PA is involved in the modulation of SCAB1 activity in F-actin reorganization.
气孔关闭相关的肌动蛋白结合蛋白 1（SCAB1）通过调节气孔保卫细胞中肌动蛋白丝的重排来调节气孔关闭。我们之前的研究表明，磷脂酰肌醇 3-磷酸（PI3P）与 SCAB1 结合并抑制其寡聚化，从而抑制其在气孔关闭过程中对 F-肌动蛋白的活性。在这项研究中，我们表明另一种磷脂，磷脂酸（PA），也与 SCAB1 结合并抑制其肌动蛋白成束活性，但不抑制其肌动蛋白结合活性。用 -丁醇处理 Col-0 幼苗可促进 F-肌动蛋白的成束， -丁醇是 PA 产生的抑制剂，但在 突变体中则没有这种作用。这些结果表明，信号分子 PA 参与了 SCAB1 在 F-肌动蛋白重排中的活性调节。
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