基于离子激流技术的新冠病毒鼻咽拭子宏转录组学研究
Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19.
作者信息
Campos Gubio S, Sardi Silvia I, Falcao Melissa B, Belitardo Emilia M M A, Rocha Danilo J P G, Rolo Carolina A, Menezes Aline D, Pinheiro Carina S, Carvalho Rejane H, Almeida João P P, Aguiar Eric R G R, Pacheco Luis G C
机构信息
Institute of Health Sciences, Federal University of Bahia, Salvador, BA, Brazil.
Núcleo de Pesquisa e Extensão em Vigilância da Saúde, Universidade Estadual de Feira de Santana, Feira de Santana, BA, Brazil.
出版信息
J Virol Methods. 2020 May 21;282:113888. doi: 10.1016/j.jviromet.2020.113888.
Herein, we describe the detection of a SARS-CoV-2 genome through metatranscriptome next-generation sequencing directly from the nasopharyngeal swab of a suspected case of local transmission of Covid-19, in Brazil. Depletion of human ribosomal RNA and use of an optimized in-house developed bioinformatics strategy contributed to successful detection of the virus.
在此，我们描述了通过宏转录组下一代测序直接从巴西一例疑似新冠病毒本地传播病例的鼻咽拭子中检测出严重急性呼吸综合征冠状病毒2（SARS-CoV-2）基因组。人核糖体RNA的去除以及使用优化的内部开发的生物信息学策略有助于成功检测到该病毒。
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