Modern Research Center for Traditional Chinese Medicine, Shanxi University, 92 Wucheng Road, 030006, Taiyuan, China.
Modern Research Center for Traditional Chinese Medicine, Shanxi University, 92 Wucheng Road, 030006, Taiyuan, China.
J Pharm Biomed Anal. 2020 Aug 5;187:113293. doi: 10.1016/j.jpba.2020.113293. Epub 2020 Apr 9.
Established qualitative analysis method for Chaigui granules based on UHPLC-Q-orbitrap-MS/MS and applied to its absorption properties studies. The LC-MS method was established to identify the structures of the components and metabolites. And then biosamples of rats after administration, e.g. intestinal solution, serum and brain microdialysate, were detected in rats with same method. Xcalibur 3.2 software was used for mass spectrum analysis and identification. Compound discover 3.0 was used for metabolite analysis. 95 chemical constituents were identified from Chaigui granules, including sesquiterpenes, flavonoids, lactones, tannins, organic acids, saponins and so on. 82 components and 11 metabolites were found in intestinal solution. 28 chemical constituents and 32 metabolites were found in serum. 15 chemical constituents were found in brain microdialysate. Vanillic acid, abiflorin, paeoniflorin, 4-hydroxybenzoic acid, lactiflorin, Z-butylidenephthalide, saikosaponin c, saikosaponin a, atractylenolide III, saikosaponin g, saikosaponin b1, sesquiterpenes, butylphthalide, saikosaponin d and glycyrrhetinic acid directly passed through the blood-brain barrier, which might be speculated that Chaigui granule plays an antidepressant role mainly through regulating brain central mechanism and endocrine mechanism, and so on. It is a systematically applicable approach for rapid identification and relative quantitation of Chaigui granules in vivo by UHPLC-Q-orbitrap-MS/MS, provides an important basis for the safety evaluation and rational clinical application of Chaigui granules.
基于 UHPLC-Q-Orbitrap-MS/MS 建立柴桂颗粒的定性分析方法及其吸收特性研究。该 LC-MS 方法用于鉴定成分和代谢物的结构。然后，用相同的方法检测大鼠给药后的生物样品，如肠液、血清和脑微透析液。Xcalibur 3.2 软件用于质谱分析和鉴定。使用 Compound Discover 3.0 进行代谢产物分析。从柴桂颗粒中鉴定出 95 种化学成分，包括倍半萜、黄酮、内酯、单宁、有机酸、皂苷等。在肠液中发现 82 种成分和 11 种代谢物。在血清中发现 28 种化学成分和 32 种代谢物。在脑微透析液中发现 15 种化学成分。香草酸、阿魏酸、芍药苷、对羟基苯甲酸、乳香内酯、Z-丁烯基邻苯二甲酸、柴胡皂苷 C、柴胡皂苷 A、苍术内酯 III、柴胡皂苷 G、柴胡皂苷 B1、倍半萜、丁基邻苯二甲酸、柴胡皂苷 D 和甘草酸直接通过血脑屏障，推测柴桂颗粒主要通过调节中枢机制和内分泌机制发挥抗抑郁作用等。这是一种通过 UHPLC-Q-Orbitrap-MS/MS 快速鉴定和相对定量柴桂颗粒体内的系统适用方法，为柴桂颗粒的安全性评价和合理临床应用提供了重要依据。
