Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.
Bor S. Luh Food Safety Research Center, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.
Molecules. 2022 Dec 29;28(1):284. doi: 10.3390/molecules28010284.
Research suggests that soyasaponins are poorly absorbed in the GI tract and that soyasaponin aglycones or soyasapogenols are absorbed faster and in greater amounts than the corresponding soyasaponins. Therefore, it is important to understand the bioavailability of these compounds for the potential development of functional foods containing their components. In this paper, to investigate the metabolic characteristics of soyasapogenols A and B, the pharmacokinetic parameters in rats were determined via oral and intravenous administration. The liver metabolites of soyasapogenols were identified using UPLC-/Q-TOF-MS/MS, and their metabolic pathways were also speculated. The results show that, after oral administration, there was a bimodal phenomenon in the absorption process. T was about 2 h, and soyasapogenol was completely metabolized 24 h later. The bioavailability of soyasapogenol was superior, reaching more than 60%. There were sixteen metabolites of soyasapogenol A and fifteen metabolites of soyasapogenol B detected in rat bile. Both phase I and II metabolic transformation types of soyasapogenols, including oxidation, dehydrogenation, hydrolysis, dehydration, deoxidization, phosphorylation, sulfation, glucoaldehyde acidification, and conjugation with cysteine, were identified. In addition, soyasapogenol A could be converted into soyasapogenols B and E in the metabolic process. These results suggest that it is feasible to use soyasapogenols as functional ingredients in nutraceuticals or food formulations.
研究表明，大豆皂甙在胃肠道中吸收不良，大豆皂甙苷元或大豆皂甙元比相应的大豆皂甙吸收更快、吸收量更大。因此，了解这些化合物的生物利用度对于开发含有其成分的功能性食品具有重要意义。在本文中，为了研究大豆皂素 A 和 B 的代谢特征，通过口服和静脉注射给药确定了大鼠的药代动力学参数。采用 UPLC-Q-TOF-MS/MS 鉴定了大豆皂素在肝脏中的代谢产物，并推测了其代谢途径。结果表明，口服后，吸收过程中存在双峰现象。T 约为 2 h，24 h 后大豆皂素完全代谢。大豆皂素的生物利用度较高，达到 60%以上。在大鼠胆汁中检测到十六个大豆皂素 A 的代谢物和十五个大豆皂素 B 的代谢物。大豆皂素的Ⅰ相和Ⅱ相代谢转化类型，包括氧化、脱氢、水解、脱水、脱氧、磷酸化、硫酸化、葡萄糖醛酸化以及与半胱氨酸的结合，均已被鉴定。此外，大豆皂素 A 可以在代谢过程中转化为大豆皂素 B 和 E。这些结果表明，大豆皂素作为功能性成分在营养保健品或食品配方中是可行的。
