Department of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Department of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; Department of Vascular Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China.
Int Immunopharmacol. 2020 Aug;85:106609. doi: 10.1016/j.intimp.2020.106609. Epub 2020 May 20.
Geniposide (GP), extracted from a traditional Chinese herb Gardenia jasminoides, has extensive pharmacological effects. But the effects and the potential mechanisms of GP on myocardial ischemia/reperfusion (I/R) injury are poorly understood. In present study, we investigated the effect of GP on myocardial I/R injury in vivo and hypoxia/reoxygenation (H/R) in vitro respectively, and its mechanism. The results showed that GP reduced myocardial infarct size, alleviated acute myocardial injury, improved cardiac function, regulated apoptosis-related proteins and inhibited apoptosis. In vitro experiments revealed that GP enhanced the cell viability, regulated apoptosis-related proteins and prevented cell apoptosis during H/R in H9c2 cells. GP inhibited the expression of autophagy-related proteins and autophagosome accumulation both in vivo and in vitro. The effects of GP were blocked by rapamycin (RAPA) administration. In summary, our results showed that GP protected against myocardial I/R injury and involved inhibition of autophagy, which might be through activating AKT/mTOR signaling pathways.
京尼平苷（GP）是从传统中药栀子中提取的，具有广泛的药理作用。但是，GP 对心肌缺血/再灌注（I/R）损伤的作用及其潜在机制尚不清楚。在本研究中，我们分别在体内和体外研究了 GP 对心肌 I/R 损伤和缺氧/复氧（H/R）损伤的作用及其机制。结果表明，GP 可减少心肌梗死面积，减轻急性心肌损伤，改善心功能，调节凋亡相关蛋白，抑制细胞凋亡。体外实验表明，GP 可增强 H9c2 细胞在 H/R 过程中的细胞活力，调节凋亡相关蛋白，防止细胞凋亡。GP 可抑制自噬相关蛋白的表达和自噬体的积累，无论是在体内还是在体外。RAPA 的给药阻断了 GP 的作用。综上所述，我们的研究结果表明，GP 可防止心肌 I/R 损伤，其作用机制可能涉及抑制自噬，这可能是通过激活 AKT/mTOR 信号通路。
