Two novel tubuliform silk gene sequences from Araneus ventricosus provide evidence for multiple loci in genome.

Spiders produce a diversity of silk fibers from multiple morphologically distinct silk glands for specific tasks, and each silk type primarily composed of one or more particular silk proteins encoded by silk gene family members believed to generated by duplication and divergence of ancient silk genes. Egg case silks spun from tubuliform glands are used to construct the tough outer structure of egg cases, are important for their reproduction. Here we present two novel complete TuSp1 sequences from orb weaving spider Araneus ventricosus. Alignment of the two spidroin iterated repeats showed both extreme intragenic homogenization. The pairwise Ka/Ks analysis revealed the terminal and repetitive regions for three TuSp1 loci including the reported TuSp1 gene are all under purifying selection. Phylogenetic analysis showed the two new TuSp1 variants could derive from recent duplication events.