International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, Shanghai, 201306, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources (Shanghai Ocean University), Ministry of Education, Shanghai, 201306, China; Fisheries and Aquaculture Programme, Department of Zoology, Quaid-i-Azam University, Islamabad, Pakistan.
International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, Shanghai, 201306, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources (Shanghai Ocean University), Ministry of Education, Shanghai, 201306, China.
Chemosphere. 2020 Oct;256:127096. doi: 10.1016/j.chemosphere.2020.127096. Epub 2020 May 16.
Global change and anthropogenic activities have driven marine environment changes dramatically during the past century, and hypoxia, acidification and warming have received much attention recently. Yet, the interactive effects among these stressors on marine organisms are extremely complex and not accurately clarified. Here, we evaluated the combined effects of low dissolved oxygen (DO), low pH and warming on the digestive enzyme activities of the mussel Mytilus coruscus. In this experiment, mussels were exposed to eight treatments, including two degrees of pH (8.1, 7.7), DO (6, 2 mg/l) and temperature (30 °C and 20 °C) for 30 days. Amylase (AMS), lipase (LPS), trypsin (TRY), trehalase (TREH) and lysozyme (LZM) activities were measured in the digestive glands of mussels. All the tested stress conditions showed significant effects on the enzymatic activities. AMS, LPS, TRY, TREH showed throughout decreased trend in their activities due to low pH, low DO, increased temperature and different combinations of these three stressors with time but LZM showed increased and then decreased trend in their activities. Hypoxia and warming showed almost similar effects on the enzymatic activities. PCA showed a positive correlation among all measured biochemical parameters. Therefore, the fitness of mussel is likely impaired by such marine environmental changes and their population may be affected under the global change scenarios.
在过去的一个世纪里，全球变化和人为活动使海洋环境发生了巨大变化，缺氧、酸化和变暖最近受到了广泛关注。然而，这些压力因素对海洋生物的相互作用影响极其复杂，尚未得到准确阐明。在这里，我们评估了低溶解氧 (DO)、低 pH 值和升温对贻贝 Mytilus coruscus 消化酶活性的综合影响。在这个实验中，贻贝暴露在 8 种处理中，包括 2 种 pH 值（8.1、7.7）、2 种 DO（6、2mg/l）和 2 种温度（30°C 和 20°C），持续 30 天。在贻贝的消化腺中测量了淀粉酶 (AMS)、脂肪酶 (LPS)、胰蛋白酶 (TRY)、海藻糖酶 (TREH) 和溶菌酶 (LZM) 的活性。所有测试的应激条件都对酶活性产生了显著影响。由于低 pH 值、低 DO 值、升高的温度以及这三种应激因素的不同组合随时间的推移，AMS、LPS、TRY 和 TREH 的活性呈持续下降趋势，但 LZM 的活性呈先增加后减少的趋势。缺氧和升温对酶活性的影响几乎相似。PCA 显示所有测量的生化参数之间存在正相关。因此，海洋环境变化可能会损害贻贝的适应性，其种群在全球变化情景下可能会受到影响。
