Kamand Morad, Ilieva Mirolyuba, Forsberg Sheena Louise, Thomassen Mads, Fex Svenningsen Åsa, Holst Bjørn, Meyer Morten, Michel Tanja Maria
Institute of Clinical Research, Department of Psychiatry Research, University of Southern Denmark, Odense, Denmark; Institute of Molecular Medicine, Department of Neurobiology Research, University of Southern Denmark, Odense, Denmark; Psychiatry in the Region of Southern Denmark, Odense University Hospital, Denmark.
Institute of Clinical Research, Department of Psychiatry Research, University of Southern Denmark, Odense, Denmark; Psychiatry in the Region of Southern Denmark, Odense University Hospital, Denmark.
Stem Cell Res. 2020 Jul;46:101834. doi: 10.1016/j.scr.2020.101834. Epub 2020 May 7.
Autism spectrum disorders are characterized by impaired social interaction and communication as well as restricted and repetitive interests and behavior. Increasing evidence points to an early-stage disruption of brain development. A human-induced pluripotent stem cell line (SDUKIi002-A) was created from skin fibroblasts from a 22-year old autistic male identified in the "FYNEN-cohort" of Southern Denmark. Reprogramming of the fibroblasts was performed using integration-free episomal plasmids. Further characterization confirmed the expression of pluripotency markers, differentiation into the three germ layers, absence of chromosomal abnormalities, and mycoplasma infection.
自闭症谱系障碍的特征是社交互动和沟通受损，以及兴趣和行为受限且重复。越来越多的证据表明大脑发育在早期就受到了干扰。从一名在丹麦南部“菲英岛队列”中被鉴定出的22岁自闭症男性的皮肤成纤维细胞中创建了一种人诱导多能干细胞系（SDUKIi002-A）。使用无整合附加体质粒对成纤维细胞进行重编程。进一步的表征证实了多能性标志物的表达、向三个胚层的分化、无染色体异常以及无支原体感染。
