Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
J Fish Biol. 2020 Aug;97(2):508-514. doi: 10.1111/jfb.14406. Epub 2020 Jun 21.
Resistin as an adipokine identified from rodents in 2001 is involved in many biological processes. However, little is known about this gene in fish. We cloned Siberian sturgeon (Acipenser baerii) resistin cDNA of 795 base pairs, encoding 107 amino acids, which showed 38-40% identity to human and rodents. Real-time quantitative PCR showed that the resistin gene was widely distributed in tissues of Siberian sturgeon, with the highest expression in liver. After fasting for 1, 3, 6 and 10 days, the expression of the resistin gene in the liver of Siberian sturgeon decreased significantly, and after refeeding on the 10 days of fasting the resistin mRNA expression increased rapidly, suggesting that resistin may play an important role in liver in response to starvation. Taken together, these results suggest that resistin may be involved in the regulation of energy homeostasis in liver.
抵抗素于 2001 年在啮齿动物中被鉴定为一种脂肪细胞因子，参与多种生物学过程。然而，关于鱼类的这种基因知之甚少。我们克隆了西伯利亚鲟（Acipenser baerii）抵抗素 cDNA，长度为 795 个碱基对，编码 107 个氨基酸，与人类和啮齿动物的同源性为 38-40%。实时定量 PCR 显示，抵抗素基因在西伯利亚鲟的组织中广泛分布，在肝脏中表达最高。经过 1、3、6 和 10 天的禁食后，西伯利亚鲟肝脏中抵抗素基因的表达显著下降，在禁食 10 天后再喂食时，抵抗素 mRNA 表达迅速增加，表明抵抗素可能在肝脏对饥饿的反应中发挥重要作用。综上所述，这些结果表明抵抗素可能参与肝脏能量稳态的调节。
