吡格列酮对电离辐射诱导的健康人淋巴细胞遗传毒性的防护作用。
Radioprotective Effect of Pioglitazone Against Genotoxicity Induced by Ionizing Radiation in Healthy Human Lymphocytes.
机构信息
Department of Pharmacy, Ramsar Campus, Mazandaran University of Medical Sciences, Ramsar, Iran.
Department of Radiopharmacy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
出版信息
Cardiovasc Hematol Agents Med Chem. 2021;19(1):72-75. doi: 10.2174/1871525718666200525005231.
OBJECTIVE
Pioglitazone (PG) is used to control high blood sugar in patients with type 2 diabetes mellitus. PG acts as a peroxisome proliferator-activated receptor γ agonist. In addition to the insulin-sensitizing effect, PG possesses anti-inflammatory effect. In this study, the protective effect of PG was evaluated against DNA damage induced by ionizing radiation in healthy human lymphocytes.
METHODS
The microtubes containing human whole blood were treated with PG at various concentrations (1-50 μM) for three hours. Then, the blood samples were irradiated with X-ray. Lymphocytes were cultured for determining the frequency of micronuclei as a genotoxicity biomarker in binucleated lymphocytes.
RESULTS
The mean percentage of micronuclei was significantly increased in human lymphocytes when exposed to IR, while it was decreased in lymphocytes pre-treated with PG. The maximum reduction in the frequency of micronuclei in irradiated lymphocytes was observed at 5 μM of PG treatment (48% decrease).
CONCLUSION
The anti-inflammatory property suggested the mechanism action of PG for protecting human lymphocytes against genotoxicity induced by ionizing radiation.
目的
吡格列酮（PG）用于控制 2 型糖尿病患者的高血糖。PG 作为过氧化物酶体增殖物激活受体 γ 激动剂。除了胰岛素增敏作用外，PG 还具有抗炎作用。在这项研究中，评估了 PG 对健康人淋巴细胞中电离辐射诱导的 DNA 损伤的保护作用。
方法
将含有人全血的微管用 PG 在不同浓度（1-50 μM）处理 3 小时。然后，用 X 射线照射血样。培养淋巴细胞以确定双核淋巴细胞中微核的频率作为遗传毒性生物标志物。
结果
当人淋巴细胞暴露于 IR 时，微核的平均百分比显著增加，而在用 PG 预处理的淋巴细胞中则减少。在用 PG 处理时，观察到照射的淋巴细胞中微核频率的最大减少（减少 48%），在 5 μM。
结论
抗炎特性表明 PG 对人淋巴细胞的作用机制是防止电离辐射引起的遗传毒性。
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