Hosseinimehr Seyed Jalal, Mahmoudzadeh Aziz, Azadbakht Mohammad, Akhlaghpoor Shahram
Department of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
Radiat Environ Biophys. 2009 Feb;48(1):95-8. doi: 10.1007/s00411-008-0190-z. Epub 2008 Sep 4.
The radioprotective effect of hawthorn (Crataegus microphylla) fruit extract was investigated in cultured blood lymphocytes from human volunteers. Peripheral blood samples were collected from five human volunteers 10 min before and 1, 2 and 3 h after a single oral ingestion of 500 mg hawthorn powder extract. At each time point, the whole blood was exposed in vitro to 150 cGy of cobalt-60 gamma irradiation, and then the lymphocytes were cultured with mitogenic stimulation to determine the micronuclei in cytokinesis-blocked binucleated cell. The lymphocytes in the blood samples collected after extract ingestion exhibited a significant decrease in the incidence of binucleated cells containing micronuclei as compared to similarly irradiated lymphocytes collected prior to extract ingestion. The maximum decrease in the frequency of micronuclei-containing cells was observed at 1 h after ingestion of Hawthorn extract (on average a 44% decrease). These data suggest that it may be possible to use Hawthorn extracts in personnel exposed to radiation in order to protect lymphocytes from radiation effects.
研究了山楂（小叶山楂）果实提取物对人类志愿者培养的血液淋巴细胞的辐射防护作用。在五名人类志愿者单次口服500毫克山楂粉提取物前10分钟以及服用后1、2和3小时采集外周血样本。在每个时间点，将全血在体外暴露于150 cGy的钴-60γ射线照射下，然后用有丝分裂刺激培养淋巴细胞，以确定胞质分裂阻滞双核细胞中的微核。与服用提取物前收集的经类似照射的淋巴细胞相比，服用提取物后收集的血样中的淋巴细胞含有微核的双核细胞发生率显著降低。摄入山楂提取物后1小时观察到含微核细胞频率的最大降幅（平均下降44%）。这些数据表明，有可能将山楂提取物用于辐射暴露人员，以保护淋巴细胞免受辐射影响。
