Zal Zohreh, Ghasemi Arash, Azizi Shima, Asgarian-Omran Hossein, Montazeri Azadeh, Hosseinimehr Seyed Jalal
Department of Radiopharmacy, Faculty of Pharmacy, Pharmaceutical Sciences Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
Department of Radiology and Radiation Oncology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Curr Radiopharm. 2018;11(2):109-115. doi: 10.2174/1874471011666180528095203.
Ionizing radiation induces DNA damage on normal cell results in apoptosis and cell deaths.
The radioprotective effects of cerium oxide nanoparticles (CNPs) on genotoxicity, apoptosis and necrosis induced by Ionizing Radiation (IR) in human healthy lymphocytes as highly radiosensitive cells were investigated.
Lymphocytes were prepared from three volunteers and then treated with CNPs at different concentrations and exposed to IR at dose 1.5 Gy. The radioprotective effects of CNPs were assessed by micronucleus (MN) assay and flow cytometry. Interleukin-1 was quantified in treated samples.
It was found that CNPs reduced the percentage of MN induced by IR in lymphocytes up to 73%. CNPs treatment significantly reduced IR-induced apoptotic and necrotic incidences in human lymphocytes. CNPs significantly reduced IL-1β produced in cell environment exposed to IR. The present study demonstrated that CNPs may be an effective radioprotector against DNA damage and apoptosis induced by IR mainly through mitigation of pro-inflammatory process in lymphocytes.
This result provides a new potential indication of CNPs for protection of normal cells during radiation therapy in the treatment of cancer or unwanted radiation exposure.
电离辐射会导致正常细胞的DNA损伤，进而引发细胞凋亡和死亡。
研究氧化铈纳米颗粒（CNPs）对作为高辐射敏感性细胞的人类健康淋巴细胞中电离辐射（IR）诱导的遗传毒性、细胞凋亡和坏死的辐射防护作用。
从三名志愿者身上制备淋巴细胞，然后用不同浓度的CNPs处理，再以1.5 Gy的剂量暴露于IR。通过微核（MN）试验和流式细胞术评估CNPs的辐射防护作用。对处理后的样本中的白细胞介素-1进行定量分析。
发现CNPs可将IR诱导的淋巴细胞中微核的百分比降低达73%。CNPs处理显著降低了IR诱导的人类淋巴细胞凋亡和坏死发生率。CNPs显著降低了暴露于IR的细胞环境中产生的白细胞介素-1β。本研究表明，CNPs可能是一种有效的辐射防护剂，主要通过减轻淋巴细胞中的促炎过程来对抗IR诱导的DNA损伤和细胞凋亡。
该结果为CNPs在癌症治疗或意外辐射暴露的放射治疗期间保护正常细胞提供了新的潜在应用指征。
