Department of Veterinary Physiology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, India.
Centre for Stem Cell Research and Regenerative Medicine, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, India.
Reprod Domest Anim. 2020 Aug;55(8):931-942. doi: 10.1111/rda.13730. Epub 2020 Jun 12.
In swine, even though the pregnant sows were with iron abundance, the inborn iron reserve of piglets was compromised. This indicates the insufficiency of molecular machinery involved in local placental iron flux. Here, we investigated the expression of iron regulatory proteins like hepcidin and ferroportin and also their association with iron reserve, inflammation and oxidative stress in placenta of full-term pregnant sows (n = 6). Amplification and sequencing of placental DNA confirmed the presence of hepcidin (MN579557) and ferroportin (MN565887) sequences and their 100% identity with existing GenBank data. Real-time amplification of placental mRNA revealed significant higher expression of hepcidin (p < .05) than ferroportin. Western blot analysis of placental tissues revealed specific bands for both hepcidin (8 kDa) and ferroportin (62 kDa) molecules. Immunohistochemistry revealed the immunoreactivity for both proteins in the cytoplasm and membrane of trophoblastic cells of the placenta. Hepcidin and ferroportin expressions were positively associated with placental non-haem iron reserve (p < .0001; p = .033), lipid peroxidation (p = .0060; p < .0001) and reactive oxygen species level (p = .0092; p = .0292). Hepcidin expression was positively associated with interleukin - 6 (p = .0002) and interferon gamma (p < .0001) expressions but ferroportin expression was negatively associated with interleukin-6 (p = .0005), interleukin-1β (p = .0226) and interferon gamma (p = .0059) expressions. This indicates hepcidin and ferroportin may have a role in controlling the local placental iron flux by acting as a molecular bridge between iron trafficking and inflammation.
在猪中，尽管妊娠母猪铁含量丰富，但仔猪的先天铁储备仍受到损害。这表明参与局部胎盘铁通量的分子机制不足。在这里，我们研究了铁调节蛋白如铁调素和亚铁转运蛋白的表达，以及它们与胎盘铁储备、炎症和氧化应激的关系，研究了足月妊娠母猪（n=6）的胎盘。胎盘 DNA 的扩增和测序证实了铁调素（MN579557）和亚铁转运蛋白（MN565887）序列的存在，并且它们与现有 GenBank 数据的 100%同一性。胎盘 mRNA 的实时扩增显示铁调素的表达显著高于亚铁转运蛋白（p<0.05）。胎盘组织的 Western blot 分析显示铁调素（8 kDa）和亚铁转运蛋白（62 kDa）分子的特异性条带。免疫组织化学显示两种蛋白在胎盘滋养层细胞的细胞质和膜中均有免疫反应性。铁调素和亚铁转运蛋白的表达与胎盘非血红素铁储备呈正相关（p<0.0001；p=0.033）、脂质过氧化（p=0.0060；p<0.0001）和活性氧水平（p=0.0092；p=0.0292）。铁调素的表达与白细胞介素-6（p=0.0002）和干扰素-γ（p<0.0001）的表达呈正相关，但亚铁转运蛋白的表达与白细胞介素-6（p=0.0005）、白细胞介素-1β（p=0.0226）和干扰素-γ（p=0.0059）的表达呈负相关。这表明铁调素和亚铁转运蛋白可能通过作为铁运输和炎症之间的分子桥梁，在控制局部胎盘铁通量方面发挥作用。
