National & Local Joint Engineering Laboratory for New Petro-chemical Materials and Fine Utilization of Resources, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China; Key Laboratory of Sustainable Resources Processing and Advanced Materials, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China; Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education, China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
National & Local Joint Engineering Laboratory for New Petro-chemical Materials and Fine Utilization of Resources, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China; Key Laboratory of Sustainable Resources Processing and Advanced Materials, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China; Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education, China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
Int J Biol Macromol. 2020 Oct 1;160:142-152. doi: 10.1016/j.ijbiomac.2020.05.142. Epub 2020 May 23.
Lignin is the second most abundant natural biomacromolecule. A new surface-modification for nano-hydroxyapatite (n-HA) by carboxymethyl β-cyclodextrin (CM-β-CD) and lignin and its reinforce effect for poly(lactide-co-glycolide) (PLGA) were investigated by Fourier transformation infrared (FTIR), X-ray diffraction pattern (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), dispersion images, the tensile tests, scanning electron microscope (SEM), differential scanning calorimeter (DSC) and polarized optical microscopy (POM), compared to the singled-modification of CM-β-CD or lignin. The results showed that the appropriate combined-modified n-HA displayed excellent synergistic effects for increasing the dispersion, yielding good interfacial bonding between n-HA with PLGA matrix. The tensile strength of the composite was still 14.53% higher than that of PLGA, for a n-HA addition amount of 15 wt%, which was significantly better than that for the singled-modified n-HA. Additionally, in vitro degradation behavior was evaluated by soaking in simulated body fluid (SBF), and their cell response was carried out by interaction tests with bone mesenchymal stem cells. The results indicated that the combined-modification method promoted good degradation behavior and apatite deposition, as well as excellent cell biocompatibility. This study may offer an important guidance to obtain PLGA-based composites reinforced by surface-modified n-HA as bone materials.
木质素是第二丰富的天然生物大分子。通过羧甲基-β-环糊精（CM-β-CD）和木质素对纳米羟基磷灰石（n-HA）进行新的表面修饰，并研究其对聚（乳酸-共-乙醇酸）（PLGA）的增强效果，采用傅里叶变换红外（FTIR）、X 射线衍射图（XRD）、透射电子显微镜（TEM）、热重分析（TGA）、分散图像、拉伸试验、扫描电子显微镜（SEM）、差示扫描量热法（DSC）和偏光显微镜（POM）进行了研究，与 CM-β-CD 或木质素的单一修饰进行了比较。结果表明，适当的组合修饰 n-HA 对提高分散性显示出优异的协同作用，在 n-HA 与 PLGA 基质之间产生良好的界面结合。当 n-HA 添加量为 15wt%时，复合材料的拉伸强度仍比 PLGA 高 14.53%，明显优于单一修饰的 n-HA。此外，通过在模拟体液（SBF）中浸泡来评估体外降解行为，并通过与骨髓间充质干细胞的相互作用试验进行细胞反应。结果表明，组合修饰方法促进了良好的降解行为和磷灰石沉积，以及优异的细胞生物相容性。这项研究可能为获得表面修饰 n-HA 增强的基于 PLGA 的复合材料作为骨材料提供重要指导。
