Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Fisheries College, Guangdong Ocean University, Zhanjiang, China.
Comp Biochem Physiol C Toxicol Pharmacol. 2020 Oct;236:108800. doi: 10.1016/j.cbpc.2020.108800. Epub 2020 May 22.
The effect of estrogens on Oryzias curvinotus juveniles were investigated by sequencing the transcriptome of O. curvinotus juveniles exposed to 17 β - estradiol for 24 h. A total of 69,071,524 and 71,210,528 raw reads were obtained for the control group (NC) and 17 β - estradiol exposure group (E2), respectively. After de novo assembly, total 133,210 unigenes were identified, and 85,837 unigenes (64.44% of 133,210) were annotated. Analysis of the transcriptome showed that exposure to 2 μg/L 17 β - estradiol led to the up-regulation of 19 genes and down-regulation of 18 genes. The eef1b and rps4x was most suitable as controls for quantitative real-time PCR (qPCR) using Reffinder. Different expression genes enrichment analysis found that exposed to 2 μg/L 17 β - estradiol affected various physiological processes, including spliceosome, phototransduction, amino sugar and nuclear sugar metabolism, hypotaurine metabolism, and renin-angiotensin system, etc. Exposing O. curvinotus juveniles to increasing concentrations of 17 β - estradiol (2 ng/L, 20 ng/L, 200 ng/L and 2 μg/L) led to significant up-regulation of vitellogenins (vtgs) and choriogenins (chgs) mRNA expression. The present study is the first high-throughput transcriptome sequencing of O. curvinotus juveniles, which will be useful for future functional analysis of genes related to environmental estrogen exposed, and development of biomarkers.
采用高通量转录组测序技术研究了 17β-雌二醇（E2）暴露对圆口铜鱼幼鱼的影响，对暴露于 17β-雌二醇 24 h 的圆口铜鱼幼鱼进行转录组测序。对照组（NC）和 17β-雌二醇暴露组（E2）分别获得了 69071524 条和 71210528 条原始读数。经过从头组装，共鉴定出 133210 条 unigenes，其中 85837 条 unigenes（133210 的 64.44%）被注释。转录组分析表明，暴露于 2μg/L 17β-雌二醇导致 19 个基因上调和 18 个基因下调。eef1b 和 rps4x 最适合作为 Reffinder 定量实时 PCR（qPCR）的对照。差异表达基因富集分析发现，暴露于 2μg/L 17β-雌二醇影响各种生理过程，包括剪接体、光转导、氨基糖和核糖代谢、高丝氨酸代谢和肾素-血管紧张素系统等。暴露于不同浓度的 17β-雌二醇（2ng/L、20ng/L、200ng/L 和 2μg/L）会导致卵黄蛋白原（vtgs）和卵黄蛋白原（chgs）mRNA 表达显著上调。本研究首次对圆口铜鱼幼鱼进行了高通量转录组测序，这将有助于对环境雌激素暴露相关基因的功能分析，并开发生物标志物。
