人类Y染色体DNA序列的组织特异性甲基化

Tissue specific methylation of human Y chromosomal DNA sequences.

作者信息

Nishioka Y

机构信息

Department of Biology, McGill University, Montreal, Quebec, Canada.

出版信息

Tissue Cell. 1988;20(6):875-80. doi: 10.1016/0040-8166(88)90028-6.

DOI:10.1016/0040-8166(88)90028-6

PMID:3245036

Abstract

This report describes two moderately repetitive human Y chromosomal DNA sequences isolated from a flow sorted Y chromosonal library. These sequences are present in XY male and XY female DNAs but absent in XX male and XX female DNAs. Genomic Southern blot analysis against DNAs isolated from different tissues showed tissue specific DNA methylation patterns. In contrast to the 2.1 kb Hae III repeats which are hypomethylated in sperm DNA, the moderately repetitive sequences used in this study are highly methylated in sperm, less methylated in blood and brain and least methylated in placental DNA.

摘要

本报告描述了从流式分选的Y染色体文库中分离出的两个中度重复的人类Y染色体DNA序列。这些序列存在于XY男性和XY女性的DNA中，但在XX男性和XX女性的DNA中不存在。对从不同组织分离的DNA进行的基因组Southern印迹分析显示出组织特异性的DNA甲基化模式。与在精子DNA中低甲基化的2.1 kb Hae III重复序列相反，本研究中使用的中度重复序列在精子中高度甲基化，在血液和大脑中甲基化程度较低，而在胎盘DNA中甲基化程度最低。