Laboratoire de Reconnaissance et Procédés de Séparation Moléculaire (RePSeM), Université de Strasbourg, Institut Pluridisciplinaire Hubert Curien (IPHC), UMR CNRS 7178, 25 rue Becquerel, 67087 Strasbourg Cedex 2, France; Laboratoire d'Etudes et de Recherche sur le Matériau Bois, EA 4370 USC INRA, Boulevard des Aiguillettes, BP 70239, 54506 Vandoeuvre lès Nancy, France.
Laboratoire de Reconnaissance et Procédés de Séparation Moléculaire (RePSeM), Université de Strasbourg, Institut Pluridisciplinaire Hubert Curien (IPHC), UMR CNRS 7178, 25 rue Becquerel, 67087 Strasbourg Cedex 2, France.
Bioresour Technol. 2020 Sep;311:123530. doi: 10.1016/j.biortech.2020.123530. Epub 2020 May 17.
Catalytic depolymerization of lignin is a challenging process due to competitive repolymerization reactions. In this paper, the oxidative depolymerization of lignin was catalyzed by a commercial laccase both in a batch experiment and in a membrane bioreactor using the same catalytic conditions. The membrane bioreactor was previously optimized to reach high permeation flux (25 L.h.m) during lignin diafiltration. While the lignin was exclusively condensed in the batch experiment leading to high molecular weight macromolecules (from 9 to 16 kDa), its depolymerization was effective in the bioreactor producing fragments of less than 1 kDa thanks to the in-situ extraction of the reaction products. This paper demonstrates that the reactor configuration is playing an essential role in triggering or preventing lignin condensation. It also reports the first proof-of-concept demonstrating that in-situ membrane extraction of the reactive fragments of lignin from the bulk medium can be useful against detrimental repolymerization reactions.
木质素的催化解聚是一个具有挑战性的过程，因为存在竞争性的重聚合反应。在本文中，木质素的氧化解聚在分批实验和使用相同催化条件的膜生物反应器中均由商业漆酶催化。之前已经对膜生物反应器进行了优化，以在木质素渗滤过程中达到高渗透通量（25 L.h.m）。虽然木质素在分批实验中仅发生缩合，导致高分子量的大分子（9 至 16 kDa），但由于反应产物的原位提取，其在生物反应器中的解聚是有效的，产生了小于 1 kDa 的片段。本文证明了反应器构型在引发或防止木质素缩合方面起着重要作用。它还首次证明了从本体介质中原位提取木质素反应片段的膜萃取对于防止有害的重聚合反应是有用的。
