曼氏血吸虫转座元件的鉴定。

Identification of Transposable Elements in Schistosoma mansoni.

机构信息

Universidade Federal do Paraná, Jandaia do Sul, Paraná, Brazil.

Departamento de Física e Ciência Interdisciplinar, Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos, São Paulo, Brazil.

出版信息

Methods Mol Biol. 2020;2151:135-144. doi: 10.1007/978-1-0716-0635-3_11.

DOI:10.1007/978-1-0716-0635-3_11

PMID:32452001

Abstract

Transposable elements (TEs) represent a significant portion of eukaryotic genomes and are important players in their dynamics and evolution. Therefore, the description of TEs and the analysis of their distribution in the genomes are important steps to understand their influence in the architecture of genomes. Here we describe the protocol used by us to identify and curate consensus TEs sequences from S. mansoni, as well the protocol to map these elements in the S. mansoni genome. We expect that these protocols may help researchers interested in studying TEs content in S. mansoni or other organisms.

摘要

转座元件（TEs）是真核生物基因组的重要组成部分，在基因组的动态和进化中起着重要作用。因此，描述 TEs 并分析它们在基因组中的分布是了解它们对基因组结构影响的重要步骤。在这里，我们描述了我们用来鉴定和整理曼氏血吸虫共识 TEs 序列的方案，以及将这些元件映射到曼氏血吸虫基因组中的方案。我们希望这些方案可以帮助那些有兴趣研究曼氏血吸虫或其他生物体中 TEs 内容的研究人员。