Duchateau Céline, Kauffmann Jean-Michel, Canfyn Michaël, Stévigny Caroline, De Braekeleer Kris, Deconinck Eric
ULB- Faculty of Pharmacy - RD3 - Pharmacognosy, Bioanalysis and Drug Discovery Unit - Bld Triomphe, Campus Plaine, Brussels, BELGIUM.
Sciensano - Scientific Direction Physical and Chemical Health Risks - Medicines and health products, Brussels, BELGIUM.
Drug Test Anal. 2020 Sep;12(9):1309-1319. doi: 10.1002/dta.2865. Epub 2020 Jul 6.
Aerial parts containing cannabidiol can be purchased in a legal way but cannabis used as recreational drug is illegal in most European countries. Δ9-tetrahydrocannabinol is one of the main cannabinoids responsible for the psychotropic effect. European Union countries and Switzerland authorize a concentration of THC of 0.2 % and 1.0 % w/w, respectively, for smoking products and industrial hemp. Public health inspectors and law enforcement officers need to check the legality of samples. Therefore there is a need for innovative approaches, allowing quality control of these products in an easy way and preferably on site. In many countries, cultivation of industrial hemp is permitted if the THC content does not exceed 0.2 % w/w. A portable equipment could be a useful measuring tool for farmers to check for the THC content at regular time. In this work, 189 samples were analysed with a benchtop and a handheld NIR device in order to create two classification methods according to European and Swiss laws. All samples were also analysed by GC-FID to determine their THC concentration. Supervised analysis was applied in order to establish the best model. For the first classification, the accuracy was 91% for the test set with the benchtop data and 93 % for the test set with the handheld data. For the second classification, the accuracies were respectively 91 % and 95 %. The obtained models, hyphenating spectroscopic techniques and chemometrics, enable to discriminate legal and illegal cannabis samples according to European and Swiss laws.
含有大麻二酚的地上部分可以合法购买，但在大多数欧洲国家，用作娱乐性毒品的大麻是非法的。Δ9-四氢大麻酚是导致精神作用的主要大麻素之一。欧盟国家和瑞士分别授权吸烟产品和工业大麻中四氢大麻酚的浓度为0.2%和1.0%（重量/重量）。公共卫生检查员和执法人员需要检查样品的合法性。因此，需要创新方法，以便以简便方式，最好是在现场对这些产品进行质量控制。在许多国家，如果四氢大麻酚含量不超过0.2%（重量/重量），则允许种植工业大麻。便携式设备可能是农民定期检查四氢大麻酚含量的有用测量工具。在这项工作中，使用台式近红外设备和手持式近红外设备对189个样品进行了分析，以便根据欧洲和瑞士法律创建两种分类方法。所有样品还通过气相色谱-火焰离子化检测器进行分析，以确定其四氢大麻酚浓度。应用监督分析以建立最佳模型。对于第一种分类，台式数据测试集的准确率为91%，手持式数据测试集的准确率为93%。对于第二种分类，准确率分别为91%和95%。所获得的将光谱技术和化学计量学相结合的模型能够根据欧洲和瑞士法律区分合法和非法大麻样品。
