Wokorach Godfrey, Otim Geoffrey, Njuguna Joyce, Edema Hilary, Njung'e Vincent, Machuka Eunice M, Yao Nasser, Stomeo Francesca, Echodu Richard
Biosciences Research Laboratory, Gulu University, P.O. Box 166, Gulu, Uganda.
Faculty of Agriculture, Gulu University, P.O. Box 166, Gulu, Uganda.
Physiol Mol Plant Pathol. 2020 Apr;110:101473. doi: 10.1016/j.pmpp.2020.101473.
is a potyvirus that infect sweet potato. The genome of the virus was analysed to understand genetic diversity, evolution and gene flow. Motifs, nucleotide identity and a phylogenetic tree were used to determine phylogroup of the isolates. Gene flow and genetic diversity were tested using DnaSP v.5. Codons evolution were tested using three methods embedded in Datamonkey. The results indicate occurrence of an isolate of phylogroup B within East Africa. Low genetic differentiation was observed between isolates from Kenya and Uganda indicating evidence of gene flow between the two countries. Four genes were found to have positively selected codons bordering or occurring within functional motifs. A motif within gene evolved differently between phylogroup A and B. The evidence of gene flow indicates frequent exchange of the virus between the two countries and gene motif provide a possible marker that can be used for mapping the distribution of the phylogroups.
是一种感染甘薯的马铃薯Y病毒属病毒。对该病毒的基因组进行了分析，以了解其遗传多样性、进化和基因流动。利用基序、核苷酸同一性和系统发育树来确定分离株的系统群。使用DnaSP v.5测试基因流动和遗传多样性。使用Datamonkey中嵌入的三种方法测试密码子进化。结果表明在东非存在B系统群的一个分离株。在肯尼亚和乌干达的分离株之间观察到低遗传分化，这表明两国之间存在基因流动的证据。发现四个基因在与功能基序相邻或位于功能基序内的密码子受到正选择。A基因内的一个基序在A系统群和B系统群之间进化方式不同。基因流动的证据表明该病毒在两国之间频繁交换，并且基因基序提供了一个可能的标记，可用于绘制系统群的分布图。
