Bacanli Merve, Anlar Hatice Gül, Başaran A Ahmet, Başaran Nursen
Hacettepe University, Faculty Of Pharmacy, Department Of Pharmaceutical Toxicology, Ankara, Turkey.
Hacettepe University, Faculty Of Pharmacy, Departments Of Pharmacognosy, Ankara, Turkey.
Turk J Pharm Sci. 2017 Aug;14(2):95-107. doi: 10.4274/tjps.07078. Epub 2017 Aug 15.
Phenolic compounds exhibit several health protective properties. Galangin, curcumin, pycnogenol, puerarin and ursolic acid are commonly used plant phenolics in folk medicine. The aim of our study was to evaluate the difference between neutral red uptake (NRU) and MTT assays using different plant phenolics (galangin, curcumin, pycnogenol, puerarin and ursolic acid) in healthy and cancer cells in different time periods.
In this study, the cytotoxic effects of these phenolic compounds were investigated by NRU and MTT assays in healthy (V79, Chinese hamster fibroblast cell line) and cancer [human cervix epithelial adenocarcinoma cell line Henrietta Lacks (HeLa) and human mammary carcinoma cell line (BT-474)] in 18, 24 and 48 h incubation periods.
Our results demonstrated that galangin, curcumin, pycnogenol, puerarin and ursolic acid decreased cell viability of V79, HeLa and BT-474 cells in a dose-dependent manner in 18, 24 and 48 h incubation periods. However, the cell survival rate was much lower in 48 h incubation period. There was no difference between the results from NRU and MTT assays.
To decide which incubation period and which cytotoxicity study to be used, the cytotoxicity mechanism of the compound must be known.
酚类化合物具有多种健康保护特性。高良姜素、姜黄素、碧萝芷、葛根素和熊果酸是民间医学中常用的植物酚类。我们研究的目的是评估在不同时间段，使用不同植物酚类（高良姜素、姜黄素、碧萝芷、葛根素和熊果酸）时，中性红摄取（NRU）和MTT法在健康细胞和癌细胞中的差异。
在本研究中，通过NRU和MTT法，在18、24和48小时的孵育期内，研究这些酚类化合物对健康细胞（V79，中国仓鼠成纤维细胞系）和癌细胞[人宫颈上皮腺癌细胞系海瑞塔·拉克斯（HeLa）和人乳腺癌细胞系（BT - 474）]的细胞毒性作用。
我们的结果表明，在18、24和48小时的孵育期内，高良姜素、姜黄素、碧萝芷、葛根素和熊果酸以剂量依赖的方式降低了V79、HeLa和BT - 474细胞的活力。然而，在48小时孵育期内细胞存活率要低得多。NRU和MTT法的结果之间没有差异。
要确定使用哪个孵育期以及哪种细胞毒性研究方法，必须了解该化合物的细胞毒性机制。
