Krizková Lívia, Chovanová Zuzana, Duracková Zdenka, Krajcovic Juraj
Institute of Cell Biology, Faculty of Natural Sciences, Comenius University, Mlynská dolina, 842 15 Bratislava, Slovak Republic.
Phytother Res. 2008 Mar;22(3):384-8. doi: 10.1002/ptr.2331.
Ofloxacin (15 microg/mL) and acridine orange (5 microg/mL) induce mutagenicity by different mechanisms in the photosynthetic flagellate Euglena gracilis. The present study examined whether Pycnogenol (PYC; 5-100 microg/mL) or Ginkgo biloba extract (EGb 761; 5-100 microg/mL) could protect against the mutagenic effects of each of the mutagens and the potential mechanisms underlying such protection. The highest concentration of PYC and EGb 761 effectively reduced the mutagenic activity of both ofloxacin and acridine orange by more than 99% (p < 0.001). Using luminol-dependent photochemical methodology it was demonstrated that EGb 761 and PYC were effective antioxidants. In addition, as determined by spectrophotometry, PYC and EGb 761 bound acridine orange. Both PYC and EGb 761 have been shown to produce dual antimutagenic effects, as evidenced by both antioxidant and physicochemical properties. The findings suggest that EGb 761 and PYC would thus be suitable for future study, not only as antioxidants, but also as antimutagenic agents.
氧氟沙星（15微克/毫升）和吖啶橙（5微克/毫升）通过不同机制在光合鞭毛虫纤细裸藻中诱发致突变性。本研究检测了碧萝芷（PYC；5 - 100微克/毫升）或银杏叶提取物（EGb 761；5 - 100微克/毫升）是否能预防每种诱变剂的诱变作用以及这种保护作用潜在的机制。PYC和EGb 761的最高浓度能有效降低氧氟沙星和吖啶橙的诱变活性超过99%（p < 0.001）。使用基于鲁米诺的光化学方法证明EGb 761和PYC是有效的抗氧化剂。此外，通过分光光度法测定，PYC和EGb 761能结合吖啶橙。PYC和EGb 761均已显示出双重抗诱变作用，抗氧化和物理化学特性均证明了这一点。研究结果表明，EGb 761和PYC因此不仅作为抗氧化剂，而且作为抗诱变剂都适合未来的研究。
