da Silva Melo P, Durán N, Haun M
Department of Biochemistry Institute of Biology, State University of Campinas (UNICAMP), CP 6110, 13081-970 SP, Campinas, Brazil.
Toxicol Lett. 2000 Aug 16;116(3):237-42. doi: 10.1016/s0378-4274(00)00226-5.
The cytotoxicity of prodigiosin, an antibiotic and potential trypanocide produced by Serratia marcescens, and Benznidazole, a trypanocidal drug, were assayed on V79 fibroblast cell line. Three independent endpoints for cytotoxicity were evaluated; namely, the nucleic acid content (NAC), MTT reduction and neutral red uptake (NRU). IC(50) values of 1-20 microM were obtained for prodigiosin in the NRU, MTT and NAC tests. Prodigiosin had greater trypanocidal activity (IC(50)=5 microM) than Nifurtimox (IC(50)=150 microM) a known trypanocide drug used in Chagas' disease therapy. Benznidazole was less toxic (IC(50)=2000 microM) than prodigiosin (IC(50)=1-20 microM) in V79 cells based on the MTT and NAC assays. Benznidazole stimulated the NRU until 2 mM. Indeed, the cell viability measured with the NRU was higher at all concentrations of benznidazole tested than that measured by MTT reduction and NAC assays.
对粘质沙雷氏菌产生的一种抗生素及潜在杀锥虫剂灵菌红素和一种杀锥虫药物苯硝唑，在V79成纤维细胞系上测定了它们的细胞毒性。评估了细胞毒性的三个独立终点；即核酸含量（NAC）、MTT还原和中性红摄取（NRU）。在NRU、MTT和NAC试验中，灵菌红素的IC50值为1 - 20微摩尔。灵菌红素具有比硝呋替莫（IC50 = 150微摩尔，一种用于恰加斯病治疗的已知杀锥虫药物）更强的杀锥虫活性（IC50 = 5微摩尔）。基于MTT和NAC测定，在V79细胞中，苯硝唑的毒性（IC50 = 2000微摩尔）低于灵菌红素（IC50 = 1 - 20微摩尔）。苯硝唑在浓度达到2毫摩尔之前会刺激NRU。实际上，在所有测试的苯硝唑浓度下，用NRU测定的细胞活力高于通过MTT还原和NAC测定的细胞活力。
