Anumolu Panikumar D, Gurrala Sunitha, Mathew Ceema, Panchakatla Vasavi, Maddala Veda
Osmania University, Gokaraju Rangaraju College of Pharmacy, Department of Pharmaceutical Analysis, Hyderabad, India.
Turk J Pharm Sci. 2019 Mar;16(1):82-87. doi: 10.4274/tjps.63634. Epub 2018 Dec 31.
A simple, rapid, specific, and highly sensitive ecofriendly spectrofluorimetric method has been developed for the quantification of praziquantel.
A linear relationship was found between fluorescence intensity and praziquantel concentration in the range of 1-20 μg/mL in water at emission wavelength of 286 nm after excitation wavelength at 263 nm with a good correlation coefficient (0.999).
The proposed method was validated according to International Conference on Harmonization guidelines and statistical analysis of the results revealed high accuracy and good precision with the percentage relative standard deviation values less than 2. The detection and quantification limits were 0.27 and 0.81 μg/mL, respectively. The proposed method was extended to investigate the stability of the drug and its degradation kinetics in the presence of acidic, alkaline, and oxidative conditions.
The method was utilized for dissolution studies of praziquantel tablet formulation. The suggested procedures could be used for the assessment of praziquantel in drug substance and drug products as well as in the presence of its degradation products.
已开发出一种简单、快速、特异且高度灵敏的环保型荧光分光光度法用于吡喹酮的定量分析。
在激发波长为263 nm、发射波长为286 nm的条件下，水中吡喹酮浓度在1 - 20 μg/mL范围内，荧光强度与吡喹酮浓度呈线性关系，相关系数良好（0.999）。
根据国际协调会议指南对所提出的方法进行了验证，结果的统计分析显示该方法具有高准确度和良好精密度，相对标准偏差百分比值小于2。检测限和定量限分别为0.27和0.81 μg/mL。所提出的方法被扩展用于研究该药物在酸性、碱性和氧化条件下的稳定性及其降解动力学。
该方法用于吡喹酮片剂制剂的溶出度研究。所建议的程序可用于原料药和药品中吡喹酮的评估，以及在其存在降解产物的情况下的评估。
