Fatemi Faezeh, Golbodagh Abbas, Hojihosseini Reza, Dadkhah Abolfazl, Akbarzadeh Kambiz, Dini Salome, Malayeri Mohammad Reza Mohammadi
Nuclear Science and Technology Research Institute, Materials and Nuclear Fuel Research School, Tehran, Iran.
Payame Noor University, Faculty of Sciences, Department of Biochemistry, Tehran, Iran.
Turk J Pharm Sci. 2020 Feb;17(1):99-107. doi: 10.4274/tjps.galenos.2018.24381. Epub 2020 Feb 19.
Natural medicine has been proposed for treating sepsis worldwide. Therefore, in this study, the effect of deuterium-depleted water (DDW) alone and adjuvant with Mill. (RD) essential oils was considered through the evaluation of oxidative stress-antioxidant parameters and the expression of cyclooxygenase-2 (COX-2) inflammatory gene in liver damage caused by sepsis.
The rats were randomly divided into 5 groups: 1) laparotomy group; 2) cecal ligation and puncture (CLP) group; 3) DDW (15 ppm and 30 ppm doses) group; 4) DDW (15 ppm and 30 ppm doses) plus RD essential oil (100 mg/kg.bw); 5) indomethacin (2 mg/kg.bw) as a positive control. The treatments were daily administrated for 2 weeks and the CLP model was created on the day 15. Then, the animals were killed and their liver tissue was separated for histopathologic and biochemical assessment.
Our results demonstrated that the treatment of animals with DDW and DDW plus RD essential oil was effective due to the regulation of the oxidative stress-antioxidant parameters including lipid peroxidation, glutathione (GSH), GSH s-transferases, myeloperoxidase, ferric reducing ability of plasma and inflammatory parameters such as prostaglandin E2 and COX-2. Pathological studies also showed that sepsis led to the liver tissue injuries, which can be reduced by treatments.
Sepsis caused oxidative stress in the liver tissue, but the administration of DDW and DDW plus RD essential oil can be useful to prevent and heal these injuries.
天然药物已被提议用于全球范围内脓毒症的治疗。因此，在本研究中，通过评估氧化应激 - 抗氧化参数以及脓毒症所致肝损伤中环氧合酶 -2（COX -2）炎症基因的表达，来考量去氘水（DDW）单独使用以及与迷迭香叶（RD）精油联合使用的效果。
将大鼠随机分为5组：1）剖腹手术组；2）盲肠结扎穿孔（CLP）组；3）DDW（15 ppm和30 ppm剂量）组；4）DDW（15 ppm和30 ppm剂量）加RD精油（100 mg/kg体重）组；5）吲哚美辛（2 mg/kg体重）作为阳性对照组。每天进行治疗，持续2周，并在第15天建立CLP模型。然后，处死动物并分离其肝脏组织用于组织病理学和生化评估。
我们的结果表明，用DDW以及DDW加RD精油治疗动物是有效的，这归因于对氧化应激 - 抗氧化参数（包括脂质过氧化、谷胱甘肽（GSH）、GSH - s -转移酶、髓过氧化物酶、血浆铁还原能力）以及炎症参数（如前列腺素E2和COX -2）的调节。病理学研究还表明，脓毒症导致肝组织损伤，而治疗可减轻这些损伤。
脓毒症在肝脏组织中引起氧化应激，但给予DDW以及DDW加RD精油有助于预防和治愈这些损伤。
