Antioxidant Activity of Different Fractions of Against Paracetamol-induced Toxicity in Rats.

OBJECTIVES

To evaluate the antioxidant activity of chloroform extract fractions of (whole plant) against paracetamolinduced toxicity in rats.

MATERIALS AND METHODS

For 7 days, rats were treated with different chloroform extract fractions and toxicity was induced with a single dose of paracetamol by intraperitoneal injection. The group of animals pretreated with 100 mg/kg p.o of fraction D of improved significantly in terms of hepatic superoxide dismutase (SOD), catalase and peroxidase activities, and glutathione levels compared to the control group.

RESULTS

The hepatic SOD, catalase, peroxidase activities, and glutathione levels in the animal groups treated with paracetamol were 33.6±0.09 μ/mg protein, 5.5±0.23 μ/mg protein, 0.131±0.15 μ/mL, and 46.1±5.81 μM, respectively. Hepatic SOD, catalase, peroxidase, and glutathione in the fraction D treated group were 61.8±0.07 μ/mg protein, 10.6±0.16 μ/mg protein, 0.913±0.23 μ/mL, and 87.6±1.4 micro molar, respectively. Therefore, the present study revealed that fraction D of has significant antioxidant activity and can be used to protect tissue from oxidative stress.

CONCLUSION

From the results, fraction D of at a dose of 100 mg/kg, p.o., improved the SOD, catalase and peroxidase activities, and glutathione levels significantly. Based on this study, we can conclude that fraction D of possesses antioxidant activity and can be employed in protecting tissue from oxidative stress.