Department of Hematology, Tianjin Medical University General Hospital, Tianjin 300052, China.
J Immunol Res. 2020 May 5;2020:6503539. doi: 10.1155/2020/6503539. eCollection 2020.
Immune-related pancytopenia (IRP) is a kind of autoimmune disease mediated by autoantibodies in bone marrow. T helper 9 (Th9) cell is a new subset of T cell discovered recently, which mainly expresses cytokine interleukin-9 (IL-9) to exert immune function. Th9 cells are associated with a variety of inflammatory diseases, but the role of Th9 cells in IRP remains unclear.
Fifty patients with IRP and 20 healthy controls were enrolled. The percentage of Th9 cells was detected by flow cytometry (FCM) and ELISA. CD4 lymphocytes were sorted by magnetic beads, and the mRNA expression levels of Th9 cells related transcription factors PU.1 and BATF were detected by RT-PCR.
The percentage of Th9 cells in CD3CD4 cells was 2.73 ± 1.96% in the untreated group, which was significantly higher than those in the remission group (1.21 ± 0.86%) ( < 0.01) and the control group (0.68 ± 0.40%) ( < 0.001). And that in the remission group was significantly higher than that in the control group ( < 0.05). The level of IL-9 in the untreated group was 183.91 ± 112.42 pg/mL, which was significantly higher than that in the remission group (105.96 ± 64.79 pg/mL) ( < 0.01) and control group (56.03 ± 14.49 pg/mL) ( < 0.001). That in the remission group was also significantly higher than that in the control group ( < 0.01). They were negatively correlated with hemoglobin, red blood cell, white blood cell, and platelet counts and positively correlated with the percentage of CD19B cells and CD5CD19/CD19B cells, respectively. The mRNA expression levels of PU.1 and BATF in IRP patients were higher than those in controls ( < 0.05).
The percentage of Th9 cells in the peripheral blood and the level of IL-9 in the serum of patients with IRP were increased, which was related to the severity of the disease.
免疫相关性全血细胞减少症（IRP）是一种由骨髓中自身抗体介导的自身免疫性疾病。辅助性 T 细胞 9（Th9）细胞是最近新发现的 T 细胞亚群，主要通过表达细胞因子白细胞介素-9（IL-9）发挥免疫功能。Th9 细胞与多种炎症性疾病有关，但 Th9 细胞在 IRP 中的作用尚不清楚。
纳入 50 例 IRP 患者和 20 名健康对照者。采用流式细胞术（FCM）和酶联免疫吸附试验（ELISA）检测 Th9 细胞的百分比。采用磁珠分选 CD4 淋巴细胞，采用 RT-PCR 检测 Th9 细胞相关转录因子 PU.1 和 BATF 的 mRNA 表达水平。
未经治疗组 CD3CD4 细胞中 Th9 细胞的百分比为 2.73±1.96%，明显高于缓解组（1.21±0.86%）（<0.01）和对照组（0.68±0.40%）（<0.001）。缓解组明显高于对照组（<0.05）。未经治疗组 IL-9 水平为 183.91±112.42pg/mL，明显高于缓解组（105.96±64.79pg/mL）（<0.01）和对照组（56.03±14.49pg/mL）（<0.001）。缓解组也明显高于对照组（<0.01）。它们与血红蛋白、红细胞、白细胞和血小板计数呈负相关，与 CD19B 细胞和 CD5CD19/CD19B 细胞的百分比呈正相关。IRP 患者的 PU.1 和 BATF mRNA 表达水平高于对照组（<0.05）。
IRP 患者外周血 Th9 细胞百分比和血清 IL-9 水平升高，与疾病严重程度有关。
