Namiki M, Nakamura M, Okuyama A, Doi Y, Matsui T, Fujisue H, Takeyama M, Fujioka H
Department of Urology, Osaka Central Hospital.
Hinyokika Kiyo. 1988 Oct;34(10):1767-70.
The influence of temperature on human Leydig and Sertoli cell functions was investigated in vitro using the organ culture technique. Specific 125I-FSH and 125I-hCG binding sites in testes from patients with prostate cancer cultured for 1, 3, 5 or 7 days at 33 degrees C or 37 degrees C were measured and compared. There were no significant differences in 125I-FSH or 125I-hCG binding sites between the two temperature groups at 1 to 7 days of culture. Testosterone concentration in the medium obtained from testicular organ culture at 33 degrees C did not differ significantly from that at 37 degrees C at 1 to 7 days of culture. In conclusion, a high temperature may not disturb the Leydig and Sertoli cell functions in a short term.
采用器官培养技术在体外研究了温度对人睾丸间质细胞和支持细胞功能的影响。对在33℃或37℃培养1、3、5或7天的前列腺癌患者睾丸中的特异性125I-FSH和125I-hCG结合位点进行了测定和比较。在培养1至7天时,两个温度组之间的125I-FSH或125I-hCG结合位点没有显著差异。在培养1至7天时,从33℃睾丸器官培养物中获得的培养基中的睾酮浓度与37℃时没有显著差异。总之,高温在短期内可能不会干扰睾丸间质细胞和支持细胞的功能。