Noknoy Royto, Sunantaraporn Sakone, Phumee Atchara, Siriyasatien Padet, Sanguansub Sunisa
Master of Science (Entomology), Department of Entomology, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen Campus, Nakhonpathom 73140, Thailand.
Vector Biology and Vector Borne Disease Research Unit, Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
Insects. 2020 May 21;11(5):318. doi: 10.3390/insects11050318.
Termites of the genus (Termitidae: Macrotermitinae) are serious agricultural and structural pests, which also play vital roles in ecosystem functioning, and are crucial for the maintenance of tropical biodiversity. They are widely distributed, mainly in Southeast Asian countries; however, the parasitism of termites has been little researched. This research was conducted to identify and study the ecology of the parasitoids of termites at Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, Thailand. (Hagen) soldier termites were collected from 25 mounds. In four of the 25 mounds, scuttle fly larvae were found inside the bodies of the soldier termites, and adult flies were found in all of the mounds. Some of the larvae successfully developed to pupae under laboratory conditions. The percentages of parasitized major soldier termites collected from the four mounds were 43.79%, 47.43%, 0.86%, and 3.49%, respectively, and the percentages of parasitized minor soldier termites were 0.64%, 0.00%, 0.21%, and 0.00%, respectively. Larvae, pupae, and adult flies were identified using both morphological and molecular identifications. Molecular identification used the partial nucleotide sequences of the mitochondrial cytochrome c oxidase I (COI) gene. The results of both identification methods identified the parasitic Diptera as the scuttle fly, (Loew) (Diptera: Phoridae). The phylogenetic analysis of the 23 scuttle fly samples (11 larvae, 7 pupae, and 5 adults) classified them into two clades: (1) Those closely related to a previous report in India; (2) those related to found in Asia and Africa. This is the first discovery of in . Further investgation into termite parasitism by and its possible use in the biological control of termites is needed.
（白蚁科：大白蚁亚科）属的白蚁是严重的农业和建筑害虫，它们在生态系统功能中也起着至关重要的作用，对维持热带生物多样性至关重要。它们分布广泛，主要分布在东南亚国家；然而，白蚁的寄生现象研究较少。本研究旨在泰国佛统府坎彭盛校区的 Kasetsart 大学鉴定和研究白蚁寄生蜂的生态学。从 25 个蚁丘中采集了（哈根）兵蚁。在 25 个蚁丘中的 4 个中，在兵蚁体内发现了蚤蝇幼虫，并且在所有蚁丘中都发现了成年蝇。一些幼虫在实验室条件下成功发育成蛹。从这四个蚁丘中采集的被寄生的大兵蚁的百分比分别为 43.79%、47.43%、0.86%和 3.49%，被寄生的小兵蚁的百分比分别为 0.64%、0.00%、0.21%和 0.00%。使用形态学和分子鉴定方法对幼虫、蛹和成年蝇进行了鉴定。分子鉴定使用了线粒体细胞色素 c 氧化酶 I（COI）基因的部分核苷酸序列。两种鉴定方法的结果都将寄生双翅目鉴定为蚤蝇，（洛伊）（双翅目：蚤蝇科）。对 23 个蚤蝇样本（11 个幼虫、7 个蛹和 5 个成虫）的系统发育分析将它们分为两个进化枝：（1）与印度先前报道密切相关的那些；（2）与在亚洲和非洲发现的相关的那些。这是在 首次发现 。需要进一步研究 对白蚁的寄生作用及其在白蚁生物防治中的可能用途。
