Clinical Psychology and Psychotherapy, Department of Psychology, University of Zurich, 8050 Zurich, Switzerland.
University Research Priority Program (URPP) Dynamics of Healthy Aging, University of Zurich, 8050 Zurich, Switzerland.
Int J Mol Sci. 2020 May 21;21(10):3654. doi: 10.3390/ijms21103654.
Background Estrogen receptor α (ERα) contributes to maintaining biological processes preserving health during aging. DNA methylation changes of ERα gene () were established as playing a direct role in the regulation of ERα levels. In this study, we hypothesized decreased DNA methylation of associated with postmenopause, lower estradiol (E2) levels, and increased age among healthy middle-aged and older women. Methods We assessed DNA methylation of promoter region from dried blood spots (DBSs) and E2 from saliva samples in 130 healthy women aged 40-73 years. Results We found that postmenopause and lower E2 levels were associated with lower DNA methylation of a distal regulatory region, but not with DNA methylation of proximal promoters. Conclusion Our results indicate that decreased methylation of cytosine-phosphate-guanine island (CpGI) shore may be associated with conditions of lower E2 in older healthy women.
雌激素受体 α (ERα) 有助于维持衰老过程中的生物过程，保持健康。ERα 基因的 DNA 甲基化变化被认为在 ERα 水平的调节中发挥直接作用。在这项研究中，我们假设与绝经后、较低的雌二醇 (E2) 水平和健康中年和老年女性年龄增加相关的 中 DNA 甲基化减少。
我们评估了来自 130 名年龄在 40-73 岁之间的健康女性的干血斑 (DBS) 和唾液样本中 ERα 基因启动子区域的 DNA 甲基化和 E2。
我们发现绝经后和较低的 E2 水平与远端调控区域的 DNA 甲基化减少相关，但与近端启动子的 DNA 甲基化无关。
我们的结果表明，在健康老年女性中，较低的雌二醇可能与 中 CpGI 岛（胞嘧啶-磷酸-鸟嘌呤岛）的甲基化减少有关。
