使用免疫球蛋白G亚类抗体鸡尾酒进行免疫染色诊断1型自身免疫性胰腺炎。
Immunostaining With Immunoglobulin G Subclass Antibody Cocktail for Diagnosis of Type 1 Autoimmune Pancreatitis.
作者信息
Nakata Rie, Uehara Takeshi, Iwaya Mai, Asaka Shiho, Kobayashi Shota, Sugano Mitsutoshi, Higuchi Kayoko, Kusama Yukiko, Nakazawa Koh, Nakaguro Masato, Kobayashi Mikiko, Tateishi Ayako, Makino Mutsuki, Kawaguchi Kenji, Maejima Toshitaka, Ishii Keiko, Sano Kenji, Shimojo Hisashi, Hori Atsushi, Otsuki Toshiaki, Hamano Hideaki, Kawa Shigeyuki, Ota Hiroyoshi
机构信息
168943Matsumoto Kyoritsu Hospital, Matsumoto, Japan.
13056Shinshu University, Matsumoto, Japan.
出版信息
Int J Surg Pathol. 2020 Dec;28(8):844-849. doi: 10.1177/1066896920924781. Epub 2020 May 26.
BACKGROUND.: Immunoglobulin (Ig) G4-related diseases (RDs) are systemic diseases in which serum IgG4 levels are frequently elevated. They can cause diffuse or focal tumor formation, organ swelling, and tissue thickening in organs infiltrated by IgG4 plasma cells. The diagnostic criteria for IgG4-RDs include an IgG4/IgG ratio >40%, but counting IgG cells can be difficult because of the weakness of IgG staining density. We hypothesized that an antibody cocktail of mixed IgG1, IgG2, IgG3, and IgG4 (AC-IgG) might give immunohistochemistry results comparable with those of IgG in IgG4-RD.
METHODS.: We compared AC-IgG reactivity with IgG expression in type 1 autoimmune pancreatitis (AIP), a representative IgG4-RD. We compared immunohistochemistry results using AC-IgG and IgG-only in 10 cases of AIP. The coefficient of variation (Cv) was used to analyze differences between AC-IgG and IgG findings in AIP by 13 board-certified pathologists.
RESULTS.: Although mean values for IgG cells did not significantly differ between AC-IgG (34.3; range = 27.4-37.1) and IgG (30.0; range = 23.0-45.6; = .6254), Cv was lower for AC-IgG (33.4%) than for IgG (51.4%; regression equation; [IgG] = 0.988 + 0.982; correlation coefficient = 0.907). The data showed that the results of both methods were largely consistent.
CONCLUSION.: AC-IgG could replace IgG to count IgG cells because of its lower Cv.
背景
免疫球蛋白（Ig）G4相关疾病（RDs）是一类全身性疾病，其血清IgG4水平常升高。它们可导致弥漫性或局灶性肿瘤形成、器官肿胀以及IgG4浆细胞浸润器官中的组织增厚。IgG4相关疾病的诊断标准包括IgG4/IgG比值>40%，但由于IgG染色密度较弱，计数IgG细胞可能存在困难。我们推测，混合IgG1、IgG2、IgG3和IgG4的抗体混合物（AC-IgG）可能在IgG4相关疾病中给出与IgG免疫组化结果相当的结果。
方法
我们比较了AC-IgG与IgG在1型自身免疫性胰腺炎（AIP）（一种典型的IgG4相关疾病）中的反应性。我们在10例AIP病例中比较了使用AC-IgG和仅使用IgG的免疫组化结果。变异系数（Cv）用于分析13名获得委员会认证的病理学家对AIP中AC-IgG和IgG结果的差异。
结果
尽管AC-IgG（34.3；范围=27.4-37.1）和IgG（30.0；范围=23.0-45.6；P=.6254）的IgG细胞平均值无显著差异，但AC-IgG的Cv（33.4%）低于IgG（51.4%；回归方程：[IgG]=0.988+0.982；相关系数=0.907）。数据表明两种方法的结果基本一致。
结论
由于AC-IgG的Cv较低，它可以替代IgG来计数IgG细胞。
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