Department of Pathophysiology, Vietnam Military Medical University, Hanoi, Vietnam.
Thai Binh Medical College, Thai Binh, Vietnam.
Viral Immunol. 2020 Sep;33(7):514-520. doi: 10.1089/vim.2020.0023. Epub 2020 May 22.
The clinical outcome of dengue is due to a complex interplay between dengue virus (DENV) and host immune factors, including complement and cytokine systems. Proinflammatory cytokines are mainly produced by monocytes in response to infectious pathogens. This study investigated the levels of proinflammatory cytokines, including tumor necrosis factor-α (TNF-α), interleukin-1 beta (IL-1β), and IL-12 in Vietnamese patients with dengue, and their correlations with the clinical outcome of dengue infection in 156 patients clinically classified as dengue without warning signs (DWS-, = 87), dengue with warning signs (DWS+, = 62), and severe dengue (SD, = 7) patients as well as in 60 healthy controls (HCs). Serum TNF-α, IL-1β, and IL-12 levels were quantified by enzyme-linked immunosorbent assay (ELISA). The results showed that TNF-α, IL-1β, and IL-12 levels were significantly increased in dengue patients compared with HCs ( < 0.0001). TNF-α levels were significantly correlated with white blood cells and platelet counts ( = 0.52, 0.2; < 0.0001, = 0.018, respectively). IL-1β levels were correlated with red blood cells counts and the levels of aspartate aminotransferase and alanine aminotransferase ( = 0.23, 0.21, 0.23; = 0.004, 0.012, 0.005, respectively). The results suggest that these three proinflammatory cytokines are associated with the clinical outcome of dengue and could play roles in the pathogenesis of the disease.
登革热的临床结果是由登革病毒（DENV）和宿主免疫因素之间的复杂相互作用引起的，包括补体和细胞因子系统。促炎细胞因子主要由单核细胞产生，以响应感染性病原体。本研究调查了来自越南登革热患者的促炎细胞因子（包括肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和白细胞介素-12）的水平，并将其与 156 例临床诊断为无预警登革热（DWS-，n=87）、有预警登革热（DWS+，n=62）和重症登革热（SD，n=7）患者以及 60 例健康对照者（HCs）的登革热感染临床结果进行相关性分析。通过酶联免疫吸附试验（ELISA）定量测定血清 TNF-α、IL-1β 和 IL-12 水平。结果显示，与 HCs 相比，登革热患者的 TNF-α、IL-1β 和 IL-12 水平显著升高（ < 0.0001）。TNF-α 水平与白细胞和血小板计数显著相关（ = 0.52，0.2； < 0.0001， = 0.018，分别）。IL-1β 水平与红细胞计数以及天冬氨酸转氨酶和丙氨酸转氨酶水平相关（ = 0.23，0.21，0.23； = 0.004，0.012，0.005，分别）。这些结果表明，这三种促炎细胞因子与登革热的临床结果相关，可能在疾病的发病机制中发挥作用。
